Abstract: Objective　To establish a dynamic cerebrospinal fluid (CSF) collection model in rhesus monkeys, providing methodological support for pharmacokinetic studies of central nervous system drugs. Methods　Three adult rhesus monkeys were selected and subjected to lumbar puncture (L3-L4/L4-L5 intervertebral space) under anesthesia. After confirming CSF outflow, an indwelling catheter was inserted and secured. During the 72-hour catheter retention period, CSF samples were dynamically collected at 0, 12, 24, 48, and 72 hours for electrolyte and routine blood tests.Results　The lumbar catheter method  enabled multiple collections at five time points, with no significant differences in routine blood parameters (WBC, RBC, HGB, etc.) across time points. Electrolyte analysis revealed a statistically significant difference only in Cl- between 0 h and 12 h (P<0.05), while other indicators (K+, Na+, etc.) remained stable.Conclusion　The lumbar puncture catheter indwelling method established in this study is characterized by its simplicity, sample stability, and high reproducibility, effectively meeting the demands of dynamic CSF collection. It provides a reliable technical solution for studying drug metabolism in the central nervous system.

Key words: Rhesus monkey, cerebrospinal fluid, dynamic collection, routine blood test, electrolytes

摘要： 目的　建立恒河猴脑脊液动态采集模型,为中枢神经系统药物动力学研究提供方法学支持。方法　选取3 只成年恒河猴,麻醉后行腰椎穿刺(L3-L4/L4-L5间隙),确认脑脊液流出后置入留置导管并固定。导管保留72 h, 于0、12、24、48、72 h动态采集脑脊液样本,检测电解质及血常规指标。结果　腰椎导管法可实现5个时间点的多 次采集,各时间点脑脊液血常规指标(白细胞、红细胞、血红蛋白浓度等)无显著差异。电解质检测显示仅Cl-在0 h 与12 h存在统计学差异(P<0.05),其余指标(K+、Na+等)保持稳定。结论　本研究建立的腰椎穿刺导管留置法具 有操作简便、样本稳定、可重复性强等特点,能有效满足脑脊液动态采集需求,为中枢神经系统药物代谢研究提供 了可靠的技术方案。

关键词: 恒河猴, 脑脊液, 动态采集, 血常规, 电解质