Abstract: Objective To evaluate the reliability of establishing a young rat model of bronchial asthma with depression behavior by solitary cage combined with ovalbumin. Method Young SD rats were randomly divided into control group, solitary control group, asthma group and solitary asthma group. The rat model of asthma with depression behavior was established by solitary cage and gregarious cage feeding combined with ovalbumin-aluminum hydroxide suspension intraperitoneal injection sensitization and ovalbumin normal saline atomization stimulation. The rat’ s weight, physical signs, lung pathology, white blood cell count and classification in venous blood, and serum content of IL-4 BDNF were observed and recorded. The behavior of rats was evaluated by sugar consumption test and forced swimming test. Result Compared with the two control groups, the weight growth of gregarious asthma group was slow ( P < 0. 05) , and the weight growth of solitary asthma group was significantly slow (P<0. 05) ; the gregarious asthma group and solitary asthma group had typical asthma like symptoms such as polypnea, scratching ear nose, the solitary asthma group had depression symptoms such as less activity and less water and food intake; the lung tissue had asthma pathological changes; the white blood cell count and lymphocyte count were increased (P<0. 05) ,compared with the two control groups and gregarious asthma group, IL-4 in serum increased ( P< 0. 05) , BDNF in serum decreased ( P< 0. 05) , sugar preference index decreased (P<0. 05) , immobility time prolonged in forced swimming test (P<0. 05) . Conclusion It is reliable to establish a young rat model of bronchial asthma with depression behavior by solitary cage combined with ovalbumin.

Key words: bronchial asthma, depression, animal model, rat

摘要： 目的 评价单笼饲养联合卵清蛋白致敏激发建立支气管哮喘伴抑郁样行为幼龄大鼠模型的可靠性。 方法 将幼龄 SD 大鼠随机分为群养对照组、孤养对照组、群养哮喘组和孤养哮喘组。 采用单笼和群笼饲养结合卵清蛋白-氢氧化铝混悬液腹腔注射致敏,卵清蛋白 0. 9% 氯化钠溶液雾化激发制备支气管哮喘伴抑郁样行为大鼠模型。观察并记录大鼠体质量、体征、肺组织病理情况、静脉血中白细胞计数及分类、血清中 IL-4 及 BDNF 水平,同时采用糖水消耗实验及强迫游泳实验进行行为学评价。 结果 与两对照组比较,群养哮喘组体质量增长缓慢( P< 0. 05) ,孤养哮喘组体质量增长明显缓慢( P< 0. 05) ;群养哮喘组及孤养哮喘组出现典型呼吸急促、抓耳挠鼻等哮喘样表现,孤养哮喘组出现活动减少、进食进水减少等抑郁样表现;肺组织出现哮喘样病理改变;全血中白细胞计数及淋巴细胞计数增高(P<0. 05) ;血清中 IL-4 升高( P< 0. 05) ;与两对照组及群养哮喘组比较,血清中 BDNF 降低( P <0. 05) ;糖水偏好指数降低(P<0. 05) ;强迫游泳实验中静止不动时间延长( P< 0. 05) 。 结论 单笼饲养结合卵清蛋白致敏激发可成功制备支气管哮喘伴抑郁样行为幼龄 SD 大鼠复合动物模型。

关键词: 支气管哮喘, 抑郁, 动物模型, 大鼠