Abstract: Objective To study the effect of costunolide (CT) on bone quality in growing rats. Method Thirty 1-month-old SD rats were randomly divided into 3 groups: control group (Control) , 9 mg / ( kg· d) CT-treated group (CT-9) and 18 mg / ( kg·d) CT-treated group ( CT-18) , body weight ( 99±3) g, 10 in each group. After 3 days of adaptive feeding, the rats were given intragastric administration, and the control group was given an equal volume of distilled water every day, weighed once a week, and detected the whole body bone mineral densities ( BMD) every 4 weeks. All rats were sacrificed after 8 weeks, and the ex vivo BMD of femurs and vertebrae were examined, micro CT scans and bone biomechanical experiments were performed. Result There was no significant difference in the body weight of the rats in each group during the experiment; at 8 weeks, the BMD of the whole body and the BMD of the isolated femur and vertebra in the CT-9 group and the CT-18 group were significantly higher than those in the Control group; The elastic modulus and maximum load in the CT-18 group were significantly higher than those in the Control group; the relative bone volume, trabecular number and volume BMD of the CT-9 group and CT-18 group were significantly higher than those of the Control group, and the degree of trabecular bone separation was significantly lower than that of the Control group. Conclusion CT can improve bone quality in growing rats, and has the potential to prevent and treat osteoporosis.

Key words: costunolide, osteoporosis, bone density, bone morphometry

摘要： 目的 研究木香烃内酯( CT) 对生长期大鼠骨质量的影响。 方法 1 月龄雌性 SD 大鼠 30 只,体质量( 99±3) g,随机分为 3 组:对照组(Control) ,9 mg / ( kg·d)的 CT 处理组(CT-9)和 18 mg / ( kg·d)的 CT 处理组(CT-18) ,每组 10 只。 适应性饲养 3 d 后,灌胃给药,Control 组每天灌服等体积蒸馏水,每周称一次体质量,每 4 周检测一次全身骨密度(BMD) 。 8 周后安乐死所有大鼠,检测股骨和椎骨离体 BMD、进行 micro CT 扫描和骨生物力学实验。结果 各组大鼠实验过程中体质量未出现统计学意义,8 周时 CT-9 组和 CT-18 组的全身 BMD 及离体股骨、椎骨BMD 均显著高于 Control 组,CT-18 组的股骨弹性模量和最大载荷显著高于 Control 组,CT-9 组和 CT-18 组的相对骨体积、骨小梁数和体积骨密度均显著高于 Control 组,骨小梁分离度显著低于 Control 组。 结论 CT 能提高生长期大鼠的骨质量,具有开发预防和治疗骨质疏松药物的潜力。

关键词: 木香烃内酯, 骨质疏松症, 骨密度, 骨形态计量学