Abstract: Objective To investigate the effect of Saussurea flavonoids capsule on the behavior of hypobaric hypoxia model mice and its anti-hypoxia mechanism. Method Mice were randomly divided into normal group, hypoxia model group, acetazolamide and Saussurea flavonoids capsule group, single lavage, after the treatment in simulated conditions of an altitude of 8 000 m for 9 h, using water maze record the change of the behavior of mice, and detection of mitochondrial membrane potential and mitochondrial complex, RT-PCR method for detection of oxygen related gene transcription, The expression of hypoxia-related genes was detected by Western blot. Result Compared with normal group, model group a prolonged latent path chaotic, dropped into the original stage, percentage of movement distance shortened, and the effective number of regional and cross platform less ( P < 0. 05 ) , mitochondrial membrane potential and mitochondrial complex I activity decreased significantly, HIF-1α, VEGF mRNA expression level increased significantly, SOD mRNA and CAT mRNA levels were significantly decreased ( P < 0. 01) . Compared with the model group, the incubation time of Saussurea flavonoids capsule group was shortened, and the times of entering effective quadrant, percentage of moving distance, times of effective area and times of crossing platform were significantly higher than those of the model group (P< 0. 05) , mitochondrial membrane potential and mitochondrial complex I activity were significantly increased ( P < 0. 01) , the mRNA expression and level of HIF-1α and VEGF were decreased, and the mRNA expression levels of SOD and CAT were increased ( P < 0. 01 ) . Conclusion Saussurea flavonoids capsule can prevent learning and memory impairment caused by hypobaric hypoxia, and the mechanism may be related to the influence of mitochondrial activity and the expression of HIF-1α, VEGF and SOD, so as to achieve a good anti-acute altitude sickness effect.

Key words: Saussurea flavone, hypobaric hypoxia mouse model, behavior, brain tissue, protective effect

摘要： 目的 探讨雪莲黄酮胶囊对低压低氧模型小鼠行为学的影响及其抗低氧作用机制。 方法 将小鼠随机分为正常组、低氧模型组、乙酰唑胺组和雪莲黄酮胶囊组,单次灌胃给药后,在模拟海拔 8 000 m 环境停留 9 h,采用水迷宫记录小鼠行为学变化情况,并检测线粒体膜电位及线粒体复合物,RT-PCR 法检测缺氧相关基因转录水平,Western blot 法检测缺氧相关基因表达情况。 结果 与正常组相比,模型组潜伏时间延长,路径杂乱无序,进入原平台次数减少、运动距离百分比缩短、有效区域次数及穿越平台次数减少(P<0. 05) ,线粒体膜电位及线粒体复合物 I 的活性显著降低,HIF-1α、VEGF mRNA 表达水平显著提高,SOD mRNA、CAT mRNA 水平显著降低( P< 0. 01) ;与模型组相比,雪莲黄酮胶囊组可缩短潜伏时间,且进入有效象限的次数、运动距离百分比、有效区域次数、穿越平台次数均显著高于模型组(P<0. 05) ,线粒体膜电位及线粒体复合物 I 的活性显著升高( P<0. 01) ,HIF-1α、VEGF mRNA表达以及水平下降,SOD、CAT mRNA 表达水平升高(P<0. 01) 。 结论 雪莲黄酮胶囊能预防低压低氧条件所造成的学习记忆障碍,其机制可能与影响线粒体活力以及 HIF-1α、VEGF、SOD 的表达有关,从而达到良好的抗急性高原病作用。

关键词: 雪莲黄酮, 低压低氧小鼠模型, 行为学, 脑组织, 保护作用