Abstract: Objective To compare two modeling method of functional bowel disorders ( FBD) in rats. Method 30 SD rats, SPF grade male, were randomly divided into three groups: normal group, modeⅠ group and model Ⅱ group. The rats in model Ⅰ were treated with bondage combined with rectal hypersensitivity stimulation, and the rats in model Ⅱ were treated with bondage combined with cold water stimulation ( Ice water mixture, 0 ℃ ) . Blood index, pathological changes of ileum and colon and intestinal thrust rate were investigated to evaluate the models. Result 1) Blood routine: There were no significant differences in WBC, RBC, Lymph and PLT between model Ⅰ group and model Ⅱ group compared with the normal group (P>0. 05) . (2) Blood biochemistry: ALT, AST, CREA, CK, Mg, P and LDH had no significant differences compared with the normal group ( P > 0. 05 ) . ( 3 ) Intestinal pushing ratio: the intestinal pushing ratio in model Ⅰ group and model Ⅱ group was significantly decreased (P<0. 05) . (4) The ileum and colon of rats in model Ⅰ group and model Ⅱ group showed basically the same pathological changes: the epithelial cells at the top of part of the ileum microvilli were detached and structurally in-complete, the epithelial cells of part of the microvilli propria were slightly edema and nuclear concentration, and the capillaries in the lamina propria were slightly dilated and hyperemic. Colonic epithelial cells were slightly swollen, lamina propria capillaries were slightly dilated and hyperemic. Eosinophilic changes were occasionally observed in some smooth muscle cells at the basic level. There were a few inflammatory cells under the lamina propria microvilli epithelial cells, and microvilli epithelial cells were occasionally shed. Conclusion The basic pathophysiological characteristics of FBD can be replicated by both method, that is, the colon and ileum show certain pathological damage, but no obvious inflammatory reaction, and the small intestine thrust rate is significantly reduced. In terms of operation procedure, model II is easier to operate in batches.

Key words: functional bowel disorder, small intestinal pushing rate, colon, ileum, animal model

摘要： 目的 比较功能性肠病大鼠模型两种造模方法。 方法 SPF 级雄性 SD 大鼠 30 只,随机分为 3 组:正常组、模型Ⅰ组、模型Ⅱ组。 模型Ⅰ组为束缚结合直肠高敏刺激法,模型Ⅱ组为束缚结合冷水( 冰水混合物,0 ℃ ) 刺激法制作功能性肠病大鼠模型。 并以血液指标、回肠和结肠的病理学变化及肠推动比来评定造模效果。 结果 1) 血常规:模型Ⅰ、Ⅱ组白细胞数(WBC) 、红细胞数(RBC) 、淋巴细胞数( Lymph) 、血小板数( PLT) 与正常组比较无显著性差异(P>0. 05) ;(2)血液生化:谷丙转氨酶(ALT) 、谷草转氨酶( AST) 、肌酐( CREA) 、肌酸激酶( CK) 、镁( Mg) 、磷( P) 、乳酸脱氢酶( LDH)与正常组相比无显著性差异( P> 0. 05) 。 ( 3) 小肠推动比率:模型Ⅰ组和模型Ⅱ组小肠推动比率与正常组比较,显著降低(P<0. 05) 。 ( 4) 模型Ⅰ组与模型Ⅱ组大鼠回肠及结肠表现出基本一致的病理变化:回肠部分微绒毛顶部上皮细胞脱落、结构不完整,部分微绒毛固有层上皮细胞轻度水肿、核浓缩,固有层中毛细血管轻度扩张充血;结肠上皮细胞轻微肿胀、固有层毛细血管轻度扩张充血,偶见基层部分平滑肌细胞嗜酸性变,微绒毛固有层上皮细胞下有少量炎性细胞,偶见微绒毛上皮细胞脱落。 结论 两种造模方法均可复制出胃肠功能障碍的基本病理生理特征,即结肠、回肠均表现出一定的病理损伤,但未出现明显的炎性反应,小肠推动率有明显降低。 两种大鼠模型均复制出胃肠功能障碍的基本病理特征,从操作方法上,当批量处理及特殊环境下造模时,推测模型Ⅱ方法操作性更高。

关键词: 功能性肠病, 小肠推动率, 结肠, 回肠, 动物模型