Abstract: Objective In order to investigate the biological differences of some physiological and biochemical indexes between SD rats and Wistar rats in SPF level at different feeding periods and different genders, and to establish the normal reference ranges of blood physiological and biochemical indexes of different strains and ages. Method Three hundred SPF Wistar rats and 300 SD rats at the age of 5 to 6 weeks old were randomly assigned into 4,12 and 24 weeks groups by weight, with half male and half female. The blood physiological and biochemical indicators were detected by fully automatic biochemical analyzer and blood cell analyzer. Result The total WBC of female rats at 12-week group and male rats at 24-week group was significantly higher than 4-week group rats ( P<0. 01) . The total WBC of male rats at 12-week group was higher than 4-week group rats ( P<0. 05) , while the total WBC in 4-week and 12-week groups female rats was lower than that in male rats ( P < 0. 05) . There was no significant difference in hematologic indexes of Wistar rats of different feeding period and genders ( P>0. 05) . The serum ALP of SD rats at 4-week group was significantly higher than that of 12-week and 24-week groups ( P<0. 01) , and the ALP of female rats at 4-week group was significantly lower than that of male rats ( P<0. 05) , and the ALP in female rats at 12-week and 24-week group was significantly lower than that in male rats ( P<0. 01) . TG in SD rats was higher than 4-week group ( P< 0. 01) . The ALP in 12-week and 24-week groups female Wistar rats was significantly higher than 4-week group rats ( P < 0. 01) , and 12 weeks male rats was significantly higher than 4 weeks rats ( P < 0. 05) . ALP of female Wistar rats at 4-week and 24-week groups was significantly higher than that of male rats ( P < 0. 01) , and ALP of female rats at 12-week group was significantly higher than that of male rats ( P< 0. 05 ) . Conclusion Different feeding periods, genders and strains of SPF rats have some distinctions in blood physiological and biochemical indexes.

Key words: SPF rats, feeding periods, genders, physiological and biochemical

摘要： 目的 考察不同饲养周期和不同性别的 SPF 级 SD 大鼠与 Wistar 大鼠的部分生理及生化指标的生物学差异,建立不同品系及不同饲养周期大鼠血液生理及生化指标的正常参考值范围。 方法 5 ~ 6 周龄 SPF 级 SD 大鼠与Wistar 大鼠各 300 只,雌雄各半,饲养于屏障环境中,随机分为饲养 4 周组、12 周组及 24 周组,并饲养相应的周数。用全自动血细胞和生化分析仪进行血液生理及生化指标检测。 结果 SD 大鼠在 12 周组雌鼠和 24 周组雌、雄鼠的白细胞计数( WBC)总数显著高于 4 周组大鼠( P<0. 01) ,12 周组雄鼠的 WBC 总数高于 4 周组大鼠(P<0. 05),4 周组和 12 周组的雌鼠 WBC 总数低于雄鼠( P < 0. 05)。 不同饲养周期和性别的 Wistar 大鼠血液学指标无显著差异(P>0. 05)。 SD 大鼠 4 周组血清碱性磷酸酶(ALP)显著高于 12 周组及 24 周组(P<0. 01),且 4 周组雌鼠 ALP 低于雄鼠(P<0. 05),12 周组和 24 周组雌鼠 ALP 显著低于雄鼠(P<0. 01);12 周组和 24 周组 SD 大鼠的甘油三酯( TG)均高于 4 周组(P<0. 05)。 Wistar 雌性大鼠 12 周组和 24 周组雌、雄鼠的 ALP 均显著高于 4 周组( P< 0. 01),12 周组雄鼠ALP 高于 4 周组(P<0. 05)。 4 周组和 24 周组 Wistar 雌鼠 ALP 显著高于雄鼠( P<0. 01),12 周组雌鼠 ALP 高于雄鼠(P<0. 05)。 结论 不同饲养周期、不同性别及不同品系的 SPF 级大鼠,血液生理及生化指标具有一定差异性。

关键词: SPF , 级大鼠, 饲养周期, 性别, 生理及生化指标