Abstract: Objective To compare the sensitivity, specificity, precision and reliability of 2 domestic and 1 imported ELISA kits for the detection of mouse hepatitis virus (MHV) antibody, so as to screen out a kind of economic and reliable ELISA kit. Method Three kinds of ELISA kits were chosen to detect MHV antibodies and other 5 kinds of viruses such as Sendai virus (SV), Peneumonia virus of mice (PVM), Minute virus of mice ( MVM), Poxvirus of mice ( MPV) and Reovirus type Ⅲ ( Reo-3) , and compared their sensitivity, specificity, precision and reliability. Result There were significant differences in the sensitivity of the 3 kinds of kits. Domestic kit B is 400 times as sensitivity as domestic kit A, and the imported kit is 4 times sensitivity of kit B. There were no cross reaction with SV, PVM, MVM, MPV and Reo-3 in domestic kit B and the imported one, but there were cross reactions with domestic kit A. The precision test showed that the average coefficient of variation in the imported kit was 5. 667%, and in the domestic kits A and B were 13. 825% and 13. 827%. The reliability test showed that the coincidence rates of positive and negative tests of domestic kit B and the imported one were both 100%, and the coincidence rates of positive and negative tests of domestic kit A were 40% and 80%, respectively. Conclusion The specficity and reliability of domestic kit B are consistent with the improted one, but with lower sensitivity and precison than it. Domestic kit A is lower in all tests than others.

Key words: ELISA kit, comparative study, mouse hepatitis virus

摘要： 目的 本研究拟对比小鼠肝炎病毒( MHV)抗体 ELISA 检测试剂盒的敏感性、特异性、精密性和可信度,以筛选出既经济又可靠的检测试剂盒。 方法 选择两种国产与一种进口的 ELISA 试剂盒检测 MHV 抗体和以下 5 种病毒阳性血清:小鼠仙台病毒(SV)、小鼠肺炎病毒(PVM)、小鼠细小病毒(MVM)、小鼠鼠痘病毒(MPV)及小鼠呼肠孤Ⅲ型病毒(Reo-3),并进行敏感性、特异性、精密性及可信度实验比较。 结果 ELISA 国产 B 试剂盒的敏感性是国产 A 试剂盒的 400 倍,进口试剂盒的敏感性是国产 B 试剂盒的 4 倍;特异性实验显示国产 A 试剂盒与 MPV 无交叉反应,但与其他 4 种病毒阳性血清均有交叉反应,国产 B 试剂盒和进口试剂盒与 MPV 以外的 5 种病毒阳性血清均无交叉反应;精密性实验显示进口试剂盒批内平均变异系数为 5. 667%,国产 A 和国产 B 试剂盒批内平均变异系数分别为13. 825%和 13. 827%;可信度实验显示国产 B 试剂盒和进口试剂盒的阳性和阴性检测符合率均为 100%,国产 A 试剂盒的阳性和阴性检测符合率分别为 40%和 80%。 结论 ELISA 检测国产 B 试剂盒除敏感性和精密度低于检测的进口试剂盒外,其特异性和可信度均良好,国产 A 试剂盒的敏感性、特异性、精密性和可信度均低于检测的进口试剂盒。

关键词: ELISA , 检测试剂盒, 比较研究, 小鼠肝炎病毒