Abstract: Abstract: Objective Standardized determination of immune function indicators and effect of cyclophosphamide on the H22 solid tumors in mice． Method H22 cells were implanted into the left axilla of 46 BALB /c mice． Tumors growth was observed 1 days later，The mice were randomly divided into tumor-bearing mice + cyclophosphamide group，tumor-bearing mice group，control mice + cyclophosphamide group，control mice group． Cyclophosphamide group intraperitoneal injection of cyclophosphamide 50mg /kg，2 times a week． Observe the general clinical manifestations，tumorigenicity，body weight，food intake，tumor volume，tumor weight，immune organ weight， blood values，blood biochemistry values，cytokines，lymphocyte transformation，NK cell activity，lymphocyte subsets and histopathology． Result Compared with normal mice，H22 tumor-bearing mice reduced food consumption，RBC，HGB，HCT significantly decreased ( P ＜ 0. 01) ，WBC，PLT，RDW significantly increased ( P ＜ 0. 01) ，GOT，GPT significantlyincreased ( P ＜ 0. 01) ，CD4 － CD8 － significantly decreased ( P ＜ 0. 01) ． The average food intake reduces the tumor-bearing mice given cyclophosphamide，D9 to D14 body weight was significantly lower ( P ＜ 0. 05) ，D5 to D14 tumor volume was significantly lower ( P ＜ 0. 01) ，tumor weight，spleen weight，thymus weight was significantly sexual lowering ( P ＜ 0. 01) ，the WBC，MCV significantly with lowering ( P ＜ 0. 01 ) ，HGB，the MCH，MCHC，the PLT significantly with increased ( P ＜ 0. 05 ) ，the GOT，GPT significantly ( P ＜ 0. 01) lower of IL － 2 significantly with reducing ( P ＜ 0. 01) ，IFN － γ significantly increased ( P ＜ 0. 01 ) ，CD4 + CD8 － ( %) ，CD4 + CD8 + ( %) ，CD4 － CD8 + ( %) ，CD3 + ( %) lymphocytes significantly with increased ( P ＜ 0. 01) ，CD4 － CD8 － ( %) lymphocytes was significantly lower ( P ＜ 0. 01) ，spleen lymphocytes transformed absorbance difference significantly lower ( P ＜ 0. 05) ，NK cell activity significantly reduce ( P ＜ 0. 05) ． Conclusion Blood parameters and immune function indicators were measured in H22 tumorbearing mice． We provide the basic data for biological response modifiers anticancer drug screening．

Key words: H22 liver cancer, solid tumors, immune function indicators, cyclophosphamide

摘要： 摘要: 目的规范化测定H22 实体瘤小鼠的免疫功能指标及环磷酰胺的影响。方法将H22 肝癌腹水瘤细胞接种 于BALB/c 小鼠左侧腋窝皮下，第2 天随机分成4 组: 荷瘤小鼠+ 环磷酰胺组、荷瘤小鼠组、正常小鼠+ 环磷酰胺 组、正常小鼠组。观察小鼠一般临床表现、成瘤情况、体质量、摄食量、肿瘤体积、肿瘤质量、免疫脏器质量、血常规 值、血生化值、细胞因子、淋巴细胞转化、NK 细胞活性、淋巴细胞亚群分类及组织病理。结果与正常小鼠比较， H22 荷瘤小鼠平均摄食量降低，RBC、HGB、HCT 显著性降低( P ＜ 0. 01) ，WBC、PLT、RDW 显著性升高( P ＜ 0. 01) ，GOT、GPT 显著性升高( P ＜ 0. 01) ，CD4 － CD8 － 显著性降低( P ＜ 0. 01) 。荷瘤小鼠给予环磷酰胺后平均摄食量降低，D9 － D14 体质量显著性降低( P ＜ 0. 05) ，D5 － D14 肿瘤体积显著性降低( P ＜ 0. 01) ，肿瘤质量、脾质量、胸腺质量显著性降低( P ＜ 0. 01) ，WBC、MCV 显著降低( P ＜ 0. 01) ，HGB、MCH、MCHC、PLT 显著增加( P ＜ 0. 05) ，GOT、GPT显著降低( P ＜ 0. 01) ， IL － 2 显著降低( P ＜ 0. 01) ， IFN － γ 显著增加( P ＜ 0. 01) ，CD4 + CD8 － ( %) 、CD4 + CD8 +( %) 、CD4 － CD8 + ( %) 、CD3 + ( %) 淋巴细胞显著增加( P ＜ 0. 01) ，CD4 － CD8 － ( %) 淋巴细胞显著降低( P ＜0. 01) ，脾淋巴细胞转化吸光度差值显著降低( P ＜ 0. 05) ，NK 细胞活性显著降低( P ＜ 0. 05) 。结论全面测定了H22 肝癌实体瘤小鼠的血液指标和免疫功能指标，为生物反应调节剂抗肿瘤药物筛选提供了基础数据。

关键词: H22 肝癌, 实体瘤, 免疫功能指标, 环磷酰胺