Abstract: Objective To establish a novel surgical model of multi-collecting cerebrospinal fluid （CSF） in Beagle dog and assess the surgical effect. Method With investigating the construction of skull of beagle dog, deciding the position of surgical point, completed the collecting process by skull puncture, collection tube covering and cerebrospinal fluid multi-drawing. Result We successfully explore a novel surgery of collecting cerebrospinal fluid, with the successful rate up to 70%. Conclusion The new model to cerebrospinal collect is favor of multi-draw CSF. It is a potential model to be popular method in colleting CSF.

Key words:  surgical model, cerebrospinal fluid, surgical position, multi-collection

摘要： 目的 通过建立一种新的手术模型，实现对脑脊液(CSF)的多次采集。方法 研究Beagle犬头颅结构，定位 手术钻孔，手术穿刺、埋管采集脑脊液。结果 动物手术存活率为80％，模型成功率为60％。结论 新模型设计 合理，有利于多次采集，此方法可成为Beagle犬脑脊液采集新手段。

关键词: 手术模型, 脑脊液, 定位, 多次采集