Objective To investigate the expression of Cre recombinase in a kind of new intestinal epithelial-specific Cre transgenic mouse. Methods

The expression of Cre recombinase in Vil1-Cre mouse could be investigated by detecting the expression of the reporter gene YFP, of which the Rosa26-YFP fluorescence reporter gene expression could be induced by Cre recombinase. Results In adult, Vil1-Cre mouse expressed Cre recombinase in the whole intestinal epithelium, including duodenum, jejunum,ileum, and colon. Meanwhile, a few scattered cells expressing Cre recombinase were observed in other tissues such as the kidney, pancreas, testis and ovary. During embryonic development, Cre recombinase is already expressed in the midgut at embryonic day 10. 5 ( E10. 5 ) , just one day later than the expression of endogenous villin protein at E9. 5. Besides, there is also a certain expression of Cre recombinase in the septum transversum, genital ridge and mesonephric tubule at E11. 5 and E12. 5.Conclusion The Vil1-Cre mouse showed a perfect expression pattern of Cre recombinase throughout the intestinal epithelium with high specificity and activity, which can be used to construct an intestinal epithelial-specific gene modified mouse model for related study.

villin, intestinal epithelial cell, tissue-specific Cre mouse, recombinant enzyme, in situ expression

目的 研究一种新的肠上皮特异性 Cre 转基因小鼠( Vil1-Cre 小鼠)其 Cre 重组酶的原位表达情况。 方法 利用 Cre 诱导表达的 Rosa26-YFP 荧光报告基因表达系统,通过检测报告基因 YFP 的表达探究 Vil1-Cre 小鼠中 Cre 重组酶的原位表达情况。 结果成体期,Vil1-Cre 小鼠的 Cre 重组酶在十二指肠、空肠、回肠以及结肠等不同部位的肠上皮中全层表达,同时在肾、胰腺、睾丸、卵巢等组织中存在少量表达。 胚胎发育阶段,胚胎期第 10. 5 天时 Cre 重组酶在中肠已有表达,比内源性 villin 蛋白胚胎期第 9. 5 天开始表达仅迟 1 d;胚胎期第 11. 5 天及第 12. 5 天时 Cre 重组酶在原始横隔、生殖嵴及中肾小管中也有表达。 结论 Vil1-Cre 小鼠中 Cre 重组酶的表达模式具有高度的肠上皮细胞特异性及表达活性,可用于构建肠上皮细胞特异性基因修饰小鼠模型及相关研究。

绒毛蛋白, 肠上皮细胞, 组织特异性 Cre 小鼠, 重组酶, 原位表达