Caging of Males and Females Together Induces Central Precocious Puberty and


	Premature Ovulation in the Ovaries of Female Mongolian Gerbils

doi:10.3969/j.issn.1006-6179.2024.05.003

Laboratory Animal Science ›› 2024, Vol. 41 ›› Issue (5): 13-18.DOI: 10.3969/j.issn.1006-6179.2024.05.003

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Caging of Males and Females Together Induces Central Precocious Puberty and

Premature Ovulation in the Ovaries of Female Mongolian Gerbils

( 1. Department of Experimental Animals,Capital Medical University, Beijing 100069,China)

( 2. Department of Experimental Zoology,School of Basic Medicine,Capital Medical University,Beijing 100069,China)

( 3. Beijing Key Laboratory of Nerve Regeneration and Repair,School of Basic Medicine,Capital Medical University,Beijing 100069,China)

Received:2023-12-23 Online:2024-10-28 Published:2024-10-28

雌雄同笼诱导雌性长爪沙鼠中枢性性早熟及卵巢的提前排卵

( 1. 首都医科大学实验动物部,北京 100069) ( 2. 首都医科大学基础医学院实验动物学学系,北京 100069)

( 3. 首都医科大学基础医学院神经再生修复研究北京市重点实验室,北京 100069)

通讯作者: 卢静( 1969—) ,教授,博士研究生导师,首都医科大学实验动物部,E-mail: lujing@ ccmu. edu. cn。仵毅( 1982—) ,副教授,首都医科大学实验动物部,E-mail: wuyi@ ccmu. edu. cn。
作者简介:池亚菲( 1987—) ,主管技师,首都医科大学实验动物部,E-mail: chiyafei@ ccmu. edu. com。
基金资助:
国家重点研发计划( 2021YFF0702805)

Abstract

Abstract:

Objective

To induce central precocious puberty and early ovulation in the ovaries of female Mongolian gerbils by applying the method of co-housing females and males immediately after whelping. Method In this study, 130 4-week-old Mongolian gerbils, including 90 females and 40 males, were selected, and the females were randomized into 9 groups, and the males were randomly divided into 4 groups of 10 each. We observed the age of female gerbils at vulval opening (VO) and the age of the first interoestrus ( D1 ) by vaginal smear; we measured body weight, ovary and uterus wet weight and calculated ovary index and uterus index; we measured the mRNA expression levels of hypothalamic gonadotropin-releasing hormone ( GnRH ) and pituitary GnRH receptor ( GnRHR ) by reverse transcription PCR; and we observed the development of follicles by HE staining. Result The average age of VO and D1 in gerbils in the experimental group was 8. 2 d and 8. 5 d earlier than that in control group 2, respectively (P<0. 05) ; the wet weight of the ovary, wet weight of the uterus, ovarian index

and uterine index in the experimental group were significantly higher than those in control group 1 and

control group 2 ( P < 0. 05 ) ; the expression of mRNA of hypothalamus GnRH-1 and pituitary GnRH

receptor in gerbils in the experimental and control groups was significantly higher than that in control

group 1; and the expression of mRNA of hypothalamic GnRH-1 and pituitary GnRH receptor in combined

cage gerbils was significantly higher than that of control group 1; compared with the uncaged gerbils, the

ovary wet weight, uterus wet weight, ovary index and uterus index were significantly different at 8 weeks

of age (P< 0. 05) , which were significantly higher in the caged group than in the uncaged group, and

there was no significant difference between the caged and uncaged groups at 9 and 10 weeks of age; the

size of the ovaries gradually increased in both the caged and uncaged groups, and the luteinization rate in

the uncaged group was 0% at 8 weeks of age to 10 weeks of age, respectively, The luteogenesis rate was

0%, 50% and 60% in the uncaged group at 8 weeks of age to 10 weeks of age, and 60%, 70% and 80%

in the caged group, respectively. Conclusion Female and male cages can induce central precocious puberty and promote ovarian ovulation in female Mongolian gerbils.

Key words:

Mongolian gerbil, single formulation, precocious puberty, hypothalamic-pituitary system, ovaries

摘要：

目的应用离乳后立即雌雄同笼饲养的方法诱导雌性长爪沙鼠中枢性性早熟及卵巢的提前排卵。方法本

研究选取 130 只 4 周龄长爪沙鼠,其中雌性 90 只,雄性 40 只,将雌性随机分为 9 组,雄性随机分为 4 组,每组 10

只。观察雌性长爪沙鼠阴门开启日龄(VO) ,阴道涂片检测第一次发情间期日龄( D1) ;测量体质量、卵巢和子宫湿

重并计算卵巢指数和子宫指数;反转录 PCR 法测定下丘脑促性腺激素释放激素 ( GnRH) 和垂体 GnRH 受体

(GnRHR) mRNA 表达水平;HE 染色,观察卵泡发育情况。结果实验组长爪沙鼠 VO 和 D1 出现的平均日龄比对

照组 2 分别提前了 8. 2 d 和 8. 5 d (P<0. 05) ;实验组卵巢湿重、子宫湿重、卵巢指数、子宫指数明显高于对照组 1 及

对照组 2(P<0. 05) ;实验组及对照组 2 沙鼠下丘脑 GnRH-1、垂体 GnRH 受体 mRNA 表达均明显高于对照组 1;合

笼长爪沙鼠与未合笼沙鼠相比较,卵巢湿重、子宫湿重、卵巢指数、子宫指数在 8 周龄时均有显著差异(P<0. 05) ,合

笼组均明显高于未合笼组,且 9 周龄和 10 周龄时无明显差异;合笼组与未合笼组卵巢大小均逐渐增大,未合笼组

8 ~ 10 周龄黄体发生率分别为 0%、50%、60%,合笼组分别为 60%、70%、80%。结论雌雄同笼可诱导雌性长爪沙

鼠中枢性性早熟并促进卵巢的提前排卵。

关键词:

长爪沙鼠, 单配制, 性早熟, 下丘脑-垂体系统, 卵巢

CLC Number:

Q95-3

CHI Yafei, PENG Boya, MA Jun , CHEN Jiannan , CHEN Bai’ an , WU Yi , LU Jin.

Caging of Males and Females Together Induces Central Precocious Puberty and

Premature Ovulation in the Ovaries of Female Mongolian Gerbils

[J]. Laboratory Animal Science, 2024, 41(5): 13-18.

池亚菲, 彭博雅, 马俊, 陈建南, 陈柏安, 仵毅, 卢静.

雌雄同笼诱导雌性长爪沙鼠中枢性性早熟及卵巢的提前排卵

[J]. 实验动物科学, 2024, 41(5): 13-18.

[1]	QIAO Yanhua, PENG Boya, CHEN Bai’ an, LU Jing. Study on Growth and Reproductive Performance of SPF Closed Colony Mongolian Gerbi#br# [J]. Laboratory Animal Science, 2024, 41(5): 9-12.
[2]	CHI Yafei , PENG Boya , MA Jun , LI Meng , CHEN Bai’ an , WU Yi , LU Jing . Development of Reproductive System of Mongolian Gerbil#br# Before and After Sexual Maturity#br# [J]. Laboratory Animal Science, 2024, 41(4): 22-28.

Caging of Males and Females Together Induces Central Precocious Puberty and

Premature Ovulation in the Ovaries of Female Mongolian Gerbils

雌雄同笼诱导雌性长爪沙鼠中枢性性早熟及卵巢的提前排卵

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