Abstract: Objective To compare the effects of three different drinking water treatments on the growth and development of SPF mice. Method 54 SPF mice were randomly divided into 3 groups. They were fed with autoclaved water, autoclaved acidified water and sterile water bag water for 90 days. Other feeding conditions were the same. During the feeding process, the routine water quality indexes, microorganisms, body weight, drinking water volume, organ weight and organ pathomorphology in drinking water were measured, and the data were analyzed by SPASS 13. 0. Result The routine indexes of drinking water quality were qualified. The sterility test of sterile water bag water and autoclaved acidified water was negative within 14 days, and the test was positive from the first day of autoclaved water; within 14 days, the daily water intake of autoclaved acidified water group was significantly lower than that of autoclaved water group ( P < 0. 01) ; compared with the autoclaved water group, the heart coefficient of the water bag group was significantly higher, the lung index was significantly lower ( P < 0. 05) , and the liver coefficient of the acidified water group was significantly higher ( P < 0. 05) ; the result of histopathological morphology showed that the organs of mice were normal without pathological changes. Conclusion Autoclaved acidified water and sterile water bag water are better than autoclaved water in bacteriostatic aspects, and sterile water bag water and autoclaved water are better than autoclaved acidified water in drinking water volume. The effects of drinking water with three different treatment method on organs and body weight were not statistically significant.

Key words: autoclaved high pressure, acidified water, sterile water bag water, SPF mice, growth and development

摘要： 目的 比较三种不同处理方式的饮用水对 SPF 级小鼠生长发育的影响。 方法 实验选用 54 只 SPF 小鼠,随机分成 3 组,分别饮用高压灭菌水、高压酸化水和无菌水袋水 90 d,其他饲养管理条件相同。 在饲养过程中对饮用水中常规水质指标、微生物以及动物体质量、饮水量、脏器重量和脏器病理形态学等进行测定,测定结果应用 SPSS13. 0 进行组间差异分析。 结果 饮水水质常规指标检测合格;14 d 内无菌水袋水和酸化水无菌检测均为阴性,高压灭菌水第 1 天起检测出现阳性;14 d 内,酸化水组日均饮水量显著低于高压灭菌水组( P< 0. 01) ;与高压灭菌水组相比,水袋水组心脏系数显著升高,肺指数显著降低 ( P<0. 05) ,酸化水组肝系数显著增高( P< 0. 05) ;脏器组织病理形态学结果显示小鼠脏器没有发生病变,为正常脏器组织。 结论 酸化水和无菌水袋在抑菌方面优于高压灭菌水,无菌水袋水和高压灭菌水在饮水量方面优于酸化水,三种不同处理方式饮用水对于脏器质量和体质量影响无统计学意义。

关键词: 高压灭菌水, 酸化水, 无菌水袋, SPF 小鼠, 生长发育