Abstract: Objective To investigate the effect of social isolation on behavioral characterization of C57BL / 6 mice. Method Sixty 2-month-old C57BL / 6 mice were randomly divided into control group and social isolation group, with 30 mice in each group, half male and half female. Mice in the control group were every 5 / cage, and mice in the social isolation group were every 1 / cage. After 8 weeks, nest building experiment, water maze experiment and open field experiment were carried out. Result Compared with the control group, the score of mice in the social isolation group was significantly lower in the nesting experiment (P<0. 05) . The times of crossing the platform, the times of entering the target quadrant, the percentage of residence time in the target quadrant and the percentage of swimming distance in the target quadrant in the social isolation group were significantly lower in the water maze experiment ( P < 0. 05 ) . The total times of crossing, the total moving distance and the average moving speed increased significantly, and the resting time decreased significantly ( P < 0. 05 ) in the open field experiment. Conclusion The executive ability of daily activities and learning and memory ability of C57BL / 6 mice decreased, and the ability of autonomous movement and inquiry behavior increased after 8 weeks of social isolation.

Key words: social isolation, ability to perform daily activities, learning and memory ability, autonomous motor ability and inquiry behavior

摘要： 目的 探讨社会隔离对 C57BL / 6 小鼠行为表征的影响。 方法 选取 60 只 2 月龄 C57BL / 6 小鼠,随机分为对照组和社会隔离组,每组 30 只,雌雄各半。 对照组 5 只 / 笼饲养,隔离组 1 只 / 笼饲养。 8 周后进行筑巢、水迷宫和旷场实验。 结果 与对照组相比,隔离组小鼠在筑巢实验中得分显著降低;在水迷宫实验中穿过平台次数、进入目标象限的次数、在目标象限停留时间百分比和在目标象限游泳距离百分比均显著降低;在旷场实验中穿格总次数、总运动距离、平均运动速度均显著增加,静止时间显著减少;上述差异均具有统计学意义( P< 0. 05) 。 结论 社会隔离 8 周的 C57BL / 6 小鼠日常活动执行能力和学习记忆能力下降,自主运动能力和探究行为增强。

关键词: 社会隔离, 日常活动执行能力, 学习记忆能力, 自主运动能力和探究行为