Abstract: Objective To explore the effect of naringin ( NG) on the intestinal flora of mice with DSS-induced colitis. Method C57BL / 6 male mice were used to construct animal models. Fifteen mice were divided into the normal group, the model group and the NG group, each group with 5 mice. Except the normal group, the other 2 groups were given 4% ( m / v) DSS as freely drunk, and the NG group was given NG for intervention from the second day. The mice were sacrificed on the 9th day, and their colon and feces were collected. Western blot was used to detect the inflammatory molecule protein p-NF-κB-p65 and the apoptosis protein B lymphama 2 ( Bcl-2) . Using PCR-denaturing gradient gel electrophoresis ( PCR-DGGE ) technology to analyze the structure of mouse intestinal flora. Result The expressions of p-NF-κB-p65 protein and Bcl-2 protein in the model group were significantly different from those in the normal group and the NG group had different degrees of changes after NG intervention. The bands in PCR-DGGE were performed by unweighted pairwise arithmetic, the UPGMA algorithm was used for cluster analysis, and the result showed that the structure of the colonic flora of mice was significantly different in each group. Conclusion NG can effectively improve the symptoms of DSS-induced colitis and improve the intestinal flora of mice.

Key words: colitis, naringin, intestinal flora

摘要： 目的 探究柚皮苷( Naringin,NG)对 DSS 诱导的结肠炎小鼠肠道菌群的影响。 方法 用 C57BL / 6 雄性小鼠构建动物模型,将 15 只小鼠分为对照组、模型组和 NG 组,每组 5 只。 除对照组外,其它两组给予 4% ( m / v) DSS 自由饮用,第 2 天给予 NG 组小鼠 NG 干预。 第 9 天处死小鼠,留取结肠和粪便。 Western blot 检测炎症分子蛋白磷酸化核因子-κB-p65( p-NF-κB-p65)和凋亡蛋白 Bcl-2。 PCR-变性梯度凝胶电泳( PCR-DGGE) 技术分析小鼠肠道菌群结构。 结果 模型组 p-NF-κB-p65 和 Bcl-2 蛋白表达与对照组比较,有显著差异( P<0. 01) ,且 NG 干预后有不同程度的改变;对 PCR-DGGE 中的条带通过非加权成对算术平均( UPGMA)算法进行聚类分析,结果显示各组小鼠结肠菌群结构具有显著差异。 结论 NG 可以有效改善 DSS 诱导的结肠炎症状,改善小鼠肠道菌群。

关键词: 结肠炎, 柚皮苷, 肠道菌群