Abstract: Objective To study the effect of high-fat diet on body weight and bone mass of rats during growing period. Method Twenty-four SD rats in 21-day-old were randomly divided into 2 groups: the general diet group and the high fat group (12 in each group, half male and half female) . After 6 weeks of feeding with the general diet or the high fat diet, the body weight was measured and the right femur was scanned by Micro-CT. The changes of body weight and bone mass were compared between these 2 groups. Result After 6 weeks of feeding, their body weight in the high fat group was significantly increased compared with that in the general diet group, and the growth rate of the body weight in the male group was significantly higher than that in the female group( P<0. 05) ; BMD was reduced, but there was statistical difference in the female group ( P < 0. 05 ) ; BV / TV and BS / TV decreased, but Tb. Sp increased ( P < 0. 05) , while BS / BV and Tb. Th did not change significantly in both rats ( P>0. 05) . Conclusion The obese rats fed with high-fat diet were more suitable to be male obese rats model, but the female obese rats were more likely to have osteoporosis and the whole loss of bone mass.

摘要:目的 研究高脂饮食对生长期大鼠体质量和骨质量的影响。 方法 24 只 21 日龄 SD 大鼠随机分为普食组和高脂组,n = 12,雌雄各半。 两组大鼠分别给予普通饲料和高脂饲料喂养 6 周后,测量体质量以及微计算机断层扫描技术( Micro-CT)扫描右侧股骨,比较两组大鼠体质量和骨质量的变化。 结果 喂养 6 周后,与普食组比较,高脂组大鼠的体质量明显增加,具有统计学差异( P<0. 05) ,且雄性大鼠体质量的增长速度高于雌性;骨密度( BMD) 降低,但仅在雌性大鼠中具有统计学差异( P<0. 05) ;骨体积分数( BV / TV)及骨表面积骨体积比( BS / TV) 降低,具有统计学意义( P<0. 05) ;骨小梁间隙( Tb. Sp)增加,结果具有统计学差异( P< 0. 05) ; 骨表面积体积比( BS / BV) 和骨小梁厚度( Tb. Th)变化不明显,无统计学差异( P>0. 05) 。 结论 高脂饲料喂养的生长期肥胖大鼠,雄性更适合作为肥胖模型动物,而雌性肥胖大鼠更容易出现骨质疏松,形成骨量整体性缺失。