Abstract: Objective To investigate the acute toxicity of Deinococcus radiodurans ( DR) and its broken products. Method The KM mice were administrated with 0. 4 mL / 10 g of 1×10⁹ / mL DR ( high concentration DR group) , 1×10⁷ / mL DR ( low concentration DR group) , 1 × 10⁹ / mL DR broken products ( high concentration DR debris group) , 1×10⁷ / mL DR broken products ( low concentration DR debris group) and Tryptone Glucose Yeast agar medium ( TGY medium group) , respectively. After intragastrically administrated twice a day every 8 hours, the mice were observed for 14 days, then the organ coefficient, body weight, blood routine ( white blood cell, lymphocyte, red blood cell, hemoglobin, hematocrit, mean corpuscular volume, platelet) and plasma biochemical indexes ( alanine aminotransferase, aspartate aminotransferase, total protein, creatinine, creatine kinase, magnesium, phosphorus, creatine kinase MB isoenzyme, lactate dehydrogenase) were used to analyze and evaluate the toxicity of DR. Result No abnormal secretion, defecation, respiration and movement were found in mice in different dose groups. From 0. 5 hour after administration to the end of the experiment, no abnormalities such as slow reaction, pupil change, eyeball protrusion, eyelid ptosis and skin color change were observed in the test mice.There were no significant differences in body weight, blood routine test and blood biochemical indexes in the trialgroups compared with TGY group. The spleen coefficient was significantly increased in the low-dose group, and the spleen pathological examination showed that there was no substantial lesion. Conclusion The live and broken DR have no acute toxicity to mice. This experiment provides experimental basis for clinical application of DR.

摘要:目的 探讨耐辐射奇球菌( Deinococcus radiodurans,DR)活菌及细菌破碎物的急性毒性。 方法 分别将 DR 高浓度 1×10⁹ / mL(高浓度活菌组) 、低浓度 1×10⁷ / mL(低浓度活菌组) 、细菌破碎物高浓度 1× 10⁹ / mL( 高浓度细菌破碎物组) 、低浓度 1×10⁷ / mL(低浓度细菌破碎物组) 以 0. 4 mL / 10 g 灌胃 KM 小鼠,TGY 培养基组灌胃相同体积的胰蛋白胨葡萄糖酵母琼脂培养基( tryptone glucose yeast agar medium) ,间隔 8 h,灌胃 2 次后,观察 14 d,以小鼠行为学、脏器系数、体质量、血常规(白细胞、淋巴细胞、红细胞、血红蛋白、红细胞压积、平均红细胞体积、血小板) 以及血浆生化指标(谷丙转氨酶、谷草转氨酶、总蛋白、肌酐、肌酸激酶、镁、磷、肌酸激酶 MB 型同工酶、乳酸脱氢酶) 变化作为评判依据,比较分析耐辐射奇球菌的毒性。 结果 不同剂量组的小鼠,均未见分泌物、大小便、呼吸及运动异常,灌胃后 0. 5 h 至实验结束,未观察到反应迟钝、瞳孔改变、眼球凸出、眼睑下垂、皮肤颜色改变等异常,受试小鼠体质量、血常规和血液生化指标与 TGY 培养基组比较,无显著差异,脾脏系数在低浓度细菌破碎物组有显著增大,病理学检查,未见实质性病变。 结论 DR 活菌及其细菌破碎物对小鼠无急性毒性,为 DR 在临床中的拓展应用提供实验依据。