Abstract: Objective To explore a method of cryopreservation and orthotopic transplantation of genetically engineering mice ovary of C57BL / 6 J background, establish a cryopreservation system. Method The ovaries from 10 d of C57BL / 6 J and three genetically engineering mice were freezed with DAP213 10 d fresh ovaries and thawed ovaries were grafed to the 4 weeks C57BL / 6 J and C57BL / 6 J( Cg) —Tyrc- 2 J / J( B6 albino) recipient, then mated with 10 weeks C57BL / 6 J male mice after 5 weeks breeding, observed offspring. Result The grafted C57BL / 6 J fresh ovaries and freeze-thawed ovaries and genetically engineering mice freeze-thawed ovaries could be restored reproduction, and gotten normal pups when mated with the same strain males. Conclusion Cryopreservation of C57BL / 6 J and genetically engineering mice ovaries with DAP213, were thawed and grafted to recipient, then mated with male, gotten normal pups. Cryopreservation of ovaries is a important method for mice resource bank.

Key words: orthotopic transplantation, ovaries, Cryopreservation, Genetically engineering mice, Mice resource bank

摘要： 目的 对 C57BL / 6 J 为背景的遗传工程小鼠卵巢冷冻及复苏后原位移植进行研究,建立遗传工程小鼠卵巢 冷冻方法,弥补小鼠资源保种体系。 方法 采用 DAP213 方法冷冻保存了 C57BL / 6 J 及 SCARB2、PSGL1、IGB3S 三 个遗传工程小鼠 10 日龄幼鼠的卵巢,将四个品系 10 日龄幼鼠的新鲜卵巢及冻存复苏卵巢原位移植到 4 周龄 C57BL / 6 J 及 C57BL / 6 J( Cg) —Tyrc- 2 J / J( B6 albino)受体雌鼠,术后饲养 5 周与 10 周龄性成熟 C57BL / 6 J 雄鼠交 配,观察移植出生幼仔。 结果 C57BL / 6 J 新鲜卵巢和冻存卵巢,三个品系遗传工程小鼠的冻存卵巢原位移植到 C57BL / 6 J 和 B6 albino 受体中,均可以恢复正常功能,交配后得到仔鼠。 结论 采用 DAP213 方法冻存 C57BL / 6 J 及以 C57BL / 6 J 为背景的遗传工程小鼠卵巢,复苏后原位移植到 C57BL / 6 J 和 B6 albino,交配后产仔,卵巢冻存方 式是小鼠资源库保存的一个重要补充。

关键词: 原位移植, 卵巢, 冷冻保存, 遗传工程小鼠, 小鼠资源库