Abstract: Rats and mice are currently the most widely used laboratory animals,and their euthanasia method have been heatedly debated.The carbon dioxide euthanasia method has the advantages of simple use,low risk and low cost.In order to make the implementation of carbon dioxide euthanasia more in line with the requirements of experimental animal welfare ethics,the application of this technology should fully consider the pressure,pain and other reactions of experimental animals.This paper reviews the evaluation system of euthanasia by inhaling carbon dioxide,the combined use of carbon dioxide and other anesthetics,and the specific implementation method of carbon dioxide euthanasia in mice and rats.The purpose of this review is to provide useful technical references to caorbon dioxide for euthanasia to mice and rats

Key words: Euthanasia, CO2, Rat, Mice

摘要： 大鼠和小鼠是目前运用最广、使用量最大的哺乳类实验动物，其安乐死方法一直备受关注。二氧化碳安乐死方法具有使用简便，危害小，低成本的优点。为了使二氧化碳安乐死的实施更加符合实验动物福利伦理的要求，应用该技术应该充分考虑实验动物的压力，疼痛等反应。鉴于此，本文综述了动物吸入二氧化碳实施安乐死的评价体系，二氧化碳与其他麻醉剂的联合使用情况以及大小鼠二氧化碳安乐死的具体实施方法及注意事项等，以期为推动该方法的实施提供有用的技术借鉴。

关键词: 安乐死, 二氧化碳, 大鼠, 小鼠