Abstract: Objective To explore and evaluate the method for the establishment of OHSS model in rats,and to provide an ideal model for study of OHSS mechanism and drug screening.Method Totally 60 Wistar and SD rats,3-5 weeks old,were randomly grouped and injected with different doses of pregnant mare serum gonadotropin (PMSG)and human chorionic gonadotropin (hCG)to establish the OHSS model.The model was evaluated by general pathology examination,measurement of viscera index,and concentration of progesterone,estrogen and VEGF in the peripheral blood.Result Among the 4 groups,SD rats that are 3 weeks old showed a greater degree of ovarian enlargement,vascular permeability enhancement,serum VEGF level increasement and other OHSS typical changes.It’s more suitable for the establishment of OHSS rat model.Conclusion A more suitable model was obtained and can lay the foundation for further study of OHSS mechanism and drugs screening.

Key words: Rat, Ovarian hyperstimulation syndrome, animal model

摘要： 目的 筛选、比较卵巢过度刺激综合征大鼠模型(OHSS)的建立方法，为OHSS机制研究及治疗药物的开发提供良好的动物模型。方法 将60只3～5周龄的Wistar和SD大鼠随机分组后注射不同剂量的孕马血清(PMSG)和人绒毛膜促性腺激素(hCG)进行OHSS模型的建立，并通过大体病理学检查、脏器指数测量、血液孕激素、雌激素及VEGF浓度测定等方法对所建立模型进行综合评价。结果 3周龄SD大鼠出现了明显的卵巢增大，血管通透性增加，血清VEGF水平上升等OHSS典型改变，更适用于进行OHSS大鼠模型的建立。结论 比较并筛选得到了可靠造模方法，为后续的OHSS发病机制研究及治疗药物开发提供了良好的动物模型。

关键词: 大鼠, 卵巢过度刺激综合征, 动物模型