Abstract: Objective To study the effects of different concentrations of tea polyphenols on intestinal flora in mice. Method Twenty four C57BL/6 mice were randomly divided into 4 groups, control group(CK, no tea polyphenols added), low-dose tea polyphenols group(LTP, 0.4 g/L GTP added in drinking water), medium dose tea polyphenols group(MTP, 0.8 g/L GTP added in drinking water), high-dose tea polyphenols(TP, 1.6 g/L GTP added in drinking water), 4 groups are given regular feed, and tea polyphenols are replaced once a day for 14 consecutive days. Analysis of intestinal microbial diversity in mice by MiSeq sequencing and bioinformatics. Result High concentration of tea polyphenols could increase the diversity of intestinal flora. The content of Lactobacillus in medium dose group and high dose group was significantly higher than that in low dose and control group(P=0.038). Conclusion Tea polyphenols can increase the diversity of intestinal microorganisms, increase the proportion of probiotics and change the composition of flora.

Key words: intestinal flora, Tea polyphenol, diversity, Lactobacillus

摘要： 目的 研究不同浓度的茶多酚对小鼠肠道菌群的影响。方法 选取24只C57BL/6小鼠，随机分为4组，对照组(CK，不添加茶多酚)、低剂量茶多酚组(LTP，饮水中添加0.4 g/L GTP)、中剂量茶多酚组(MTP，饮水中添加0.8 g/L GTP)、高剂量茶多酚组(TP，饮水中添加1.6 g/L GTP)，4组均给予常规饲料，茶多酚饮水每天更换一次，连续14 d。经MiSeq 测序和生物信息学分析小鼠肠道微生物多样性。结果 高浓度茶多酚摄入可增加肠道菌群的多样性，中剂量组和高剂量组中乳酸杆菌的含量明显高于低剂量和对照组(p值=0.038)，茶多酚对乳杆菌的增殖效果最为明显并且呈剂量依赖性。结论 茶多酚具有可以增加肠道微生物的多样性，增加益生菌的比例及改变菌群组成的功能。

关键词: 肠道菌群, 茶多酚, 多样性, 乳杆菌