Abstract: Objective To study the behavioral and NF-κB expression changes in welfare impaired rats caused by restrain-stress. Method Wrap restraint stress (WRS) were administrated by using tubular container within male SD rats (± 200 g). At time points of 0, 1, 3, 7, 10 and 14 days after WRS administration, open filed test and elevated o-maze test were employed to analyze the physical behavior changes of SD rats. NF-κB p65 mRNA transcription levels and protein expression in all SD rats were examined as well. Result Significant expression enhancements were observed both in NF-κB p65 mRNA transcription and NF-κB p65 protein expression at the 3rd day after WRS administration compared with pre-procedure (P<0.05), but no behavior changes could be found. At the 7th day after WRS administration, significant decline (P<0.05) in physical activity capacity and activity intension were noticed. All SD rats exhibited symptoms of depression and dread. Conclusion Changes in NF-κB mRNA and protein expression may prior to behavioral changes in welfare impaired rats.

Key words: animal welfare, restrain-stress, behavior

摘要： 目的 观察制动致福利受损SD大鼠部分行为学改变以及体内NF-κB的变化。方法 选取体质量约200 g的雄性SD大鼠，使用管状容器给予束缚应激刺激，并通过旷场实验和O迷宫观测大鼠在第0、1、3、7、10、14天的行为学表现。测定了相应时期的NF-κB p65 mRNA转录水平和蛋白表达量。结果 束缚第3天起，大鼠肝脏组织NF-κB p65 mRNA转录水平和蛋白表达均较束缚前显著增高 (P< 0.05)，但此时尚未见行为学改变；束缚第7天起大鼠的活动兴趣与活动能力水平较束缚前显著下降 (P< 0.05)，显示焦虑、恐惧状态。结论 制动致福利受损大鼠肝细胞内NF-κB mRNA及蛋白表达的变化早于行为学的改变。

关键词: 动物福利, 束缚, 行为学