Abstract: Objective To investigate the effect of SCD1 gene deletion on organ coefficients and hematology indexes of mice. Method Organ coefficients are observed in 8 weeks SCD1-/- mice and heterozygous SCD1mice as well as hematology indexes of different ages mice are compared between the two group. Result Liver coefficient of 6-8 weeks SCD1-/- mice markedly increase compared with heterozygous mice. RBC,MPV,PCT of SCD1-/- mice are significantly lower (P<0.05),while MCV,MCH,RDW,PDW,PLT,LYM are remarkbly higher(P<0.05) than those of the control group. ALB,TC,TG also obviously go down and ALP,ALT,UA clearly go up(P<0.01)in 6-8 weeks experiment group. MCHC, PLT, PCT,ALP, TC of 16-18 weeks significantly decrease compared with those of SCD1+/- mice. At the same time,RDW、HCT and MCV significantly increases(P<0.01). Conclusion SCD1 gene deletion impairs the organ coefficients of mice. Moreover, gene SCD1 deletion has impact on some hematology indexes.

Key words: SCD1mice, organ coefficients, hematology indexes

摘要： 目的 探讨SCD1基因缺失对小鼠脏器参数，血液生理生化指标的影响。方法 选取8周龄SCD1-/-小鼠及其杂合子SCD1+/-小鼠，称取体质量及主要脏器，观察其脏器系数。比较不同周龄SCD1-/-小鼠及其杂合子SCD1+/-小鼠的血液细胞学和生化指标，观察其变化特点。结果 8周龄SCD1-/-小鼠与杂合子小鼠比较肝脏系数显著升高(P<0.05)。6～8周龄SCD1-/-小鼠与杂合子小鼠的血液细胞学指标相比RBC、MPV、PCT显著降低(P<0.05)，MCV、MCH、RDW、PDW、PLT、LYM显著增高(P<0.05)；血清生化指标ALB、TC、TG极显著降低(P<0.01)，ALP、ALT、UA极显著增高(P<0.01)。16～18周龄SCD1-/-小鼠与杂合子小鼠的血液细胞学指标MCHC、PLT、PCT相比极显著降低(P<0.01)，RDW、HCT、MCV都极显著增高(P<0.01)；血清生化指标ALP、TC极显著降低(P<0.01)。结论 SCD1基因缺失对小鼠鼠脏器参数、血液生理生化指标都有一定的影响。

关键词: SCD1-/-小鼠, 脏器系数, 血液生理生化指标