Abstract: To investigate the localization and expression of NADH dehydrogenase 3 (ND3) and nuclear factor κB (NF-κB) in livers and kidneys of spontaneous hereditary diabetic and non-diabetic gerbils. We selected 6 diabetic and 6 non-diabetic control gerbils, and collected livers and kidneys from each animal, respectively. Immunohistochemistry was conducted to localize and semi-quantify ND3 and NF-κB in diabetic and non-diabetic control groups. Both ND3 and NF-κB were expressed in hepatocytes. And compared with the control group, NF-κB expression in livers was elevated significantly in the diabetic group. ND3 was also localized in renal tubular epithelial cells of kidneys. And ND3 was up-regulated in kidneys of diabetic gerbils, relative to the control gerbils. In conclusion , ND3 expressed in renal tubular epithelial cells was significantly up-regulated in kidneys of diabetic gerbils, and NF-κB expressed in hepatocytes was also increased in livers of diabetic gerbils, suggesting that the aberrant expression of ND3 in kidneys and NF-κB in livers may relate with the initiation and development of diabetes in gerbils.

Key words: Mongolian gerbils Type 2 diabetes, ND3, NF-κB, Immunohistochemistry

摘要： 的 比较正常血糖长爪沙鼠和自发遗传性糖尿病长爪沙鼠肝脏和肾脏组织中烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位3(NADH dehydrogenase 3，ND3)和核转录因子κb(nuclear factor κB，NF-κB)蛋白的表达定位差异。方法 选取正常血糖长爪沙鼠和自发遗传性糖尿病长爪沙鼠各6只，分别采集动物的肝脏和肾脏组织，利用免疫组织化学检测ND3、NF-κB在各组织中的定位和表达水平。结果 ND3、NF-κB表达于长爪沙鼠肝脏实质细胞胞质中，与对照动物相比，糖尿病长爪沙鼠肝脏中NF-κB表达显著升高；ND3还表达于长爪沙鼠肾脏肾小管上皮细胞胞质中，且与对照动物相比，糖尿病长爪沙鼠肾脏中ND3表达显著升高。结论 在糖尿病长爪沙鼠中，ND3显著高表达于肾脏肾小管上皮细胞，NF-κB显著高表达于肝实质细胞，提示肾脏中ND3和肝脏中NF-κB的异常升高可能参与长爪沙鼠糖尿病的发生和发展。

关键词: 长爪沙鼠, 2型糖尿病, ND3, NF-κB, 免疫组织化学