Abstract: Objective Though detecting the pathological changes of laboratory rodents in Beijing, it provided a basis for the quality monitoring and improvement of laboratory rodents in Beijing. Method We collected hearts, livers, spleens, lungs, kidneys and intestinal tracts from 400 laboratory rodents in Beijing including 180 rats, 80 mice, 120 guinea pigs and 20 golden hamsters, and then made the paraffin sections stained with HE. The samples of the hepatocellular swelling were also stained by PAS and oil red O staining made from frozen sections, and observed under the microscope. Result The lesions of laboratory rodents mainly were hepatocellular swelling of mice, rats, guinea pigs and glycogen deposition in different degree, and liver fatty degeneration in guinea pigs; In addition, the mice and golden hamsters, especially hamsters, showed more liver necrosis and inflammatory cell infiltration. And the heart of mice and guinea pigs were hyperplasia, inflammatory cells infiltrated of kidney could be seen in mice. Conclusion The vast majority of laboratory rodents were pathologically diagnosed as healthy animals. But the feeding and management of some rodents still need to be strengthened, to provide a reliable and effective premise for the accuracy of the research experiment.

Key words: laboratory rodents, pathological diagnosis, pathological changes

摘要： 目的 通过检测北京市啮齿类实验动物的病理变化，为北京市实验动物质量监控及改进提供依据。方法 对送检的400只啮齿类实验动物，其中包括180只小鼠、80只大鼠、120只豚鼠及20只金黄地鼠，采集心、肝、脾、肺、肾、大肠和小肠，制成石蜡切片后进行HE染色，对镜检肝细胞肿胀的样品又分别进行了PAS染色和冰冻切片油红O染色，并于显微镜下观察。结果 此次进行实验动物质量的抽检，检出病变主要为小鼠、大鼠、豚鼠均出现不同程度的肝脏细胞肿胀，并均有糖原沉积病变检出，脂肪变性在豚鼠检出；此外小鼠及金黄地鼠，尤其是部分金黄地鼠出现明显肝脏坏死及炎性细胞浸润；小鼠和豚鼠的心脏发生增生性炎；小鼠肾脏炎性细胞浸润。结论 本次抽检的啮齿类实验动物基本健康，部分啮齿类动物的饲养管理仍需加强，本监测结果可为科研实验的准确性提供可靠有效的依据。

关键词: 啮齿类实验动物, 病理检测, 病变