Abstract: Abstract: To compare the advantages and disadvantages between different dosage and methods of administration of cortisone in deficiency of Kidney Yang animal models，we used intragastric administration，intraperitoneal injection and subcutaneous injection ways to mice with different doses ( 12. 5，25，50 mg / kg) of hydro-cortisone for 9 days to investigate the variations on mortality rate，general state，weight，anus temperature，autonomic activities，swimming duration and viscera index． After 9 days，general status in mice is poorer，the 25，50 mg / kg dose group are more obvious． Besides subcutaneous injection of 25 mg / kg group，other groups weight was significantly lower than before building; besides intragastric and intraperitoneal injection of 12. 5 mg / kg，subcutaneous injections of 25 mg / kg group，number of autonomic activities in mice decreased to some extent; in addition to the various ways of 12. 5 mg / kg group，the swimming time was significantly shortened than the blank group; intraperitoneal injection and subcutaneous injection of 25 mg / kg group spleen index，all ways of 12. 5 mg / kg group of adrenal gland index compared with the blank group has no obvious change，the rest of the group of spleen，thymus and adrenal gland index were significantly reduced，seminal vesicle and preputial glands of each dose group have no obvious change． So we synthesized the inspection indexes，intragastric administration 25 mg / kg dose group is superior to that of other groups． All the indexes can show the signs and symptoms of Kidney Yang Deficiency，it is more reliable Kidney Yang Deficiency model method．

Key words: <p>Kidney Yang Deficiency, mice, animal model, hydro cortisone</p>

摘要： 摘要: 目的 考察不同给药方式、不同剂量氢化可的松肾阳虚造模方法的优劣。方法 采用灌胃、腹腔注射及皮下注射的方式给予小鼠不同剂量( 12. 5、25、50 mg / kg 体质量) 氢化可的松注射液 9 d，考察小鼠的死亡率、一般状态、体质量、肛温、自主活动、脏器指数及游泳时间的变化。结果 造模 9 d 后，小鼠懒动，消瘦，拱背蜷曲，体毛枯萎无光泽，精神萎靡，反应迟钝，肛周污染，便溏等，以 25、50 mg / kg 剂量组较为明显。除皮下注射 25 mg / kg 组 外，其他各组体质量均较造模前明显下降; 除灌胃及腹腔注射 12. 5 mg / kg、皮 下 注 射 25 mg / kg组外，小鼠自主活动次数均有不同程度的减少; 除各方式的 12. 5 mg / kg 组外，小鼠游泳时间均较空白组明显缩短; 腹腔注射和皮下注射 25 mg / kg 组脾指数、各方式的 12. 5 mg / kg 组的肾上腺指数与空白组相比无明显变化，其余 各 组 的 脾、胸腺及肾上腺指数均明显减轻，各剂量组的精囊腺和包皮腺无明显变化。结论 综合各考察指标，灌胃给药 25 mg / kg 剂量组成模率较高，且各项指标均可呈现出肾阳虚的症状和体征，是比较可靠的肾阳虚造模方法。

关键词: <, p>, 肾阳虚, 小鼠, 动物模型, 氢化可的松<, /p>