Abstract: Abstract: Objective Using the thyleneglycol( EG) -based solutions with two steps to freeze the 2-cell embryos of Mongolian gerbil( Meriones unguiculatus) and to appraise the effect of embryo cryopreservation． Method The embryos were exposed serially to EFS20 for 3 minutes and EFS40 for 1 minute． The embryo trealed were immersed into liquid nitrogen． Result The embryo recovery rate was 86. 73% and after freezing-thawing it survival rate was 82. 94% ． And about 30% of the freezing-thawing embryos developed to blastocyst in vitro． Conclusion This results demonstrates that Mongolian gerbil embryos can be safely cryopreserved using vitrification solutions based on EG with two steps．

Key words: vitrification, cryopreservation, 2-cell embryo, Mongolian gerbil( Meriones unguiculatus )

摘要： 摘要: 目的 评价用乙二醇玻璃化冷冻液二步法对长爪沙鼠 2-细胞胚胎冷冻保存的效果。方法 通过超排卵处理 后与雄鼠交配获得长爪沙鼠 2-细胞胚胎，胚胎在冷冻液 EFS20 中平衡 3 min，然后移入冷冻液 EFS40 中平衡 1 min， 之后将细胞冻存管直接浸入液氮中保存。采用二步法复苏胚胎，复苏后移入改良的胚胎培养液( mKSOM) 中体外 培养。结果 采用该方法冷冻的胚胎复苏后胚胎回收率为 86. 73% ，存活率为 82. 94% ，复苏后存活的胚胎中约 1 / 3 胚胎可以在体外发育到囊胚。结论 乙二醇玻璃化冷冻液适于冷冻保存长爪沙鼠 2-细胞胚胎。

关键词: 玻璃化冷冻, 冷冻保存, 2-细胞胚胎, 长爪沙鼠