Abstract: Abstract: Objective To observe the effect of Kv1. 5、Kv2. 1 channels in rat pulmonary arterial smooth cells on the process of pulmonary vasoconstriction induced by 15-KETE． Method Western blot and PCＲ were used to identify the effect of 15-KETE on expression of Kv1. 5、Kv2. 1 channels in cultured rat pulmonary arterial smooth muscle cells ( PASMCs) ． Ｒesult ( 1) The expressions of Kv1. 5 mＲNA and protein in rat PASMCs were significantly down-regulated by 15-KETE． ( 2) Theexpressions of Kv2. 1 mＲNA and protein in rat PASMCs were significantly down-regulated by 15- KETE． Conclusion Kv1. 5、Kv2. 1 channels are involved in vasoconstriction induced by 15-KETE in rat PA．

Key words: 15-ketoeicosatetretraenoic acid, hypoxic pulmonary vasoconstriction, voltage-gated potassium channel
subtype, pulmonary arterial smooth cells

摘要： 摘要: 目的从细胞分子水平研究电压门控型钾通道亚型Kv1. 5、Kv2. 1 在15-酮基二十碳四烯酸( 15- ketoeicosatetretraenoic acid， 15-KETE) 致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD 大鼠肺动脉 平滑肌细胞( pulmonary arterial smooth cells，PASMCs) ，使用ＲT-PCＲ 和Western blot 技术观察15-KETE 对大鼠 PASMCs 上Kv1. 5、Kv2. 1 通道表达的影响。结果( 1) 15-KETE 下调大鼠PASMCs 膜上Kv1. 5 通道mＲNA 及蛋白 质的表达; ( 2) 15-KETE 下调大鼠PASMCs 膜上Kv2. 1 通道mＲNA 及蛋白质的表达。结论Kv1. 5、Kv2. 1 通道参 与由15-KETE 引起的缺氧肺动脉收缩( hypoxic pulmonary vasoconstriction，HPV) 。

关键词: 15-酮基二十碳四烯酸, 缺氧肺动脉收缩, 电压门控性K + 通道亚型, 肺动脉平滑肌细胞