Abstract: Abstract: Objective To investigate the effect of storage time on blood routine test of venous blood sample in SD rats at room temperature． Methods Sixteen SD rats( 200 ～ 220) g were selected ，which female and male was half and half，which were adopted Venous blood ． Venous blood samples were detected at 0 min，30 min，1 h，2 h，4 h， 8 h，24 h and 48 h respectively at room temperature( 18 ～ 25℃) by using MEK-6318K automatic blood cell analyzer．We compared the effect of different time on blood cells and blood platelet in the same venous blood sample． Results The HCT and LYM% at 48 hours were significantly higher than those at 0 min ( P ＜ 0. 05) ． The MCV at 24 hours and 48 hours were very significantly higher than those at 0 minute ( P ＜ 0. 01 ) ． Comparing the RDW and GRN% at 0 minute，the difference was significant ( P ＜ 0. 05) at 24 hours and very significant ( P ＜ 0. 01) at 48 hours． In contrast with 0 minute，the PLT at different time was significantly higher than the one at 0 minute ( P ＜ 0. 01) ． Comparing the PCT at 0 minute，the difference was significant ( P ＜ 0. 05) at 30 min、1 h、2 h and 4 h， very significant ( P ＜ 0. 01) at other times． The difference of MID was significant ( P ＜ 0. 05) at 24 hours and 48 hours compared to the one at 0 minute． Other hematological parameters in different periods showed no significant differences ( P ＞ 0. 05 ) ． Conclusion At room temperature，the blood routine test of venous blood in SD rats should be detected within 30 minutes to 24 hours．

Key words: SD rats, venous blood, storage time, blood routine test

摘要： 摘要: 目的探讨SD 大鼠静脉血样本在室温下放置时间对血常规测定数据的影响。方法16 只SD 大鼠( 200 ～ 220) g，雌雄各半，采静脉血经EDTA-K2 抗凝，置室温( 18 ～ 25℃ ) ，分别于0 min，30 min，1 h，2 h，4 h，8 h，24 h 和 48 h 用MEK-6318K 型全自动血液分析仪进行测定。比较不同保存时间对同一静脉血样本血细胞及PLT 的影响。 结果雌雄SD 大鼠血常规数据变化趋势一致，HCT 在48 h 和0 min 比较差异有统计学意义( P ＜ 0. 05) ; MCV 在 24 h、48 h 和0 min 比较差异有统计学意义( P ＜ 0. 01) ; RDW 24 h 和0 min 比较差异有统计学意义( P ＜ 0. 05) ，48 h 和0 min 比较差异有统计学意义( P ＜ 0. 01 ) ; PLT 各时间段和0 min 比较差异有统计学意义( P ＜ 0. 01 ) ; PCT 30 min、1 h、2 h、4 h 和0 min 比较差异有统计学意义( P ＜ 0. 05) ，其它时间段和0 min 比较差异有统计学意义( P ＜ 0. 01) ; LYM%48 h 和0 min 比较差异有统计学意义( P ＜ 0. 05) ; MID% 24 h、48 h 和0 min 比较差异有统计学意义 ( P ＜ 0. 05) ; GRN% 24 h 和0 min 比较差异有统计学意义( P ＜ 0. 05) ; GRN% 48 h 和0 min 比较差异有统计学意义 ( P ＜ 0. 01) ; 其他指标比较差异无统计学意义( P ＞ 0. 05) 。结论SD 大鼠静脉血在常温条件下放置，血常规最好 在30 min 以后，24 h 以内进行测定。

关键词: SD 大鼠, 静脉血, 放置时间, 血常规检测