Abstract: Abstract: For the past several decades，ELISA and IFA have been used as the primary assays for viral or bacterial antibody detection． However，these traditional methods are either time-consuming or labor-intensive． Microspherebased suspension array technologies， such as the Luminex xMAP  system， offer a new platform for highthroughput biomolecule detection which has been used in a variety of research fields． Using Luminex  Corporation’s xMAP  technology， the Charles River Laboratories has developed Multiplexed Fluorometric ImmunoAssayTM ( MFIATM ) profiles for sero-surveillance of various laboratory animal species，which has promoted the level of laboratory animal quality control significantly．

Key words: fluorescent microsphere, laboratory animal quality control

摘要： 摘要: ELISA 和IFA 是实验室主要的血清学检测方法，在过去的数十年中得到广泛的应用。但其存在着操作繁琐、 耗时长的问题。美国Luminex 公司开发的xMAP 是一种快速、高通道的技术平台，可在同一反应孔内同时检测上 百种抗原、抗体、核苷酸片段、受体/配体等生物分子，在医学界已广泛应用。MFIATM 是美国Charles River Laboratories 应用xMAP 平台开发的应用于实验动物质量控制的多通路、高效率的抗体检测技术，该技术的应用大 大改善了实验动物质量控制的水平。

关键词: 流式荧光, 微球技术, 实验动物质量控制