Abstract: Objective To observe the effect of stability and tolerance of the Ⅱdiabetes mellitus SD rats model.Methods SD rats are injected intraperitoneally with 25 mg /kg streptozotocin once after fed high fat diet 4 weeks.Results when the SD rats were injected STZ one week,the blood glucose of SD rats were stably higher then 6.78 mmol /L.During the 22 days observation,Although the blood glucose values of SD rats have fluctuated at a latter stage,the stability and tolerated of blood glucose of SD rats were well,were over 6.78 mmol /L.Conclusion The Ⅱdiabetes rats model can be established successfully with feeding high fat diet and injecting a low dose STZ(25 mg/kg) once.This method is simple and use a small amount of medication,the model has hatter stability and tolerance.

Key words: Ⅱ Diabetes mellitus, SD rats model, Stability and Tolerance

摘要： 目的探讨Ⅱ型糖尿病SD大鼠模型的稳定性与耐受性。方法采用连续4周喂养高脂饲料后,空腹一次性注射25 mg/kg链脲佐菌素的方法,建立Ⅱ型糖尿病SD大鼠模型。结果造模7 d后血糖值均高于6.78 mmol/L。持续观察22 d,血糖值后期略有波动,但稳定性与耐受性良好(均高于6.78 mmol/L)。结论采用高脂饮食,联合空腹一次性注射25 mg/kg链脲佐菌素的方法能成功建立Ⅱ型糖尿病SD大鼠模型。该方法建立模型简单、用药量小,有良好的稳定性与耐受性。

关键词: Ⅱ型糖尿病, SD大鼠模型, 稳定性, 耐受性