Abstract: Objective To evaluate the efficacy,the physical disinfection method of ozone and UV was studied in the animal surgery laboratory,in which the main direction is organ transplantation of animal liver,heart and kidney.Methods In an animal clean room,where there is a day-night cycle of operation and rest,the efficacy of disinfection and sterilization was evaluated.Using culture utensils and surface microbe inspections,we compared the physical disinfection methods of ozone and UV to the chemical method of 2% Glutaral.Both sterilization processes were conducted during the night.Results Both methods achieved a normal level of sterilization after three times.However,the ozone is easy to isolate,safe to use and does not require washing.Conclusion Both Ozone and UV are physical factors.They are a good alternative to the chemical sterilization methods,as there are no waste products Also ozone is able to thoroughly permeate all parts of the equipment,including the hard to reach interior.As the needs of organ transplantation research increase,large numbers of equipment and materials need to be kept in the lab for long periods of time.Therefore it is important to establish an effective disinfection method.Our results suggest that at the correct dosage of ozone and UV,we can achieve good sterilization results in the animal surgery laboratory.

Key words: Experimental animal, Surgery laboratory, Physical sterilization(ozone), Chemical sterilization

摘要： 目的观察以肝、心、肾等器官移植为主的医学实验动物手术室,使用臭氧和紫外线的物理学方法灭菌的效果。方法在实验室运转后,用物理的臭氧和紫外灭菌与化学的2%戊二醛灭菌方法进行比较,用平皿菌落记数和物品表面微生物检测,重复消毒灭菌和检测各3 d,观测消毒灭菌效果。结果臭氧的泄漏不多,易于隔离和应用。两种方法的平皿菌落记数和物品表面微生物检测均达标。结论臭氧和紫外线物理灭菌方法在完成消毒后无残留物质,可进入缝隙,在实验动物手术室等存放大量仪器物品的实验室,用物理方法替代化学方法值得提倡。

关键词: 实验动物, 手术室, 物理灭菌(臭氧), 化学灭菌