Abstract: Objective To study the effects caused by α-tocopheryl succinate(α-TOS) on cell growth and expression of IAPs (inhibitors of apoptosis proteins) in human breast cancer MCF7 and MDA-MB-453 cell lines.Methods MTT assay was used to measure the cell proliferation inhibition rates induced by α-TOS.Human breast cancer MCF7 and MDA-MB-453 cells were inoculated in 96-well plates,and then were treated with α-TOS(5,10,25,50,75,100,125 and 150 μmol/L),48 h later,the proliferation inhibition rates were measured by MTT assay.Western blotting and RT-PCR were performed to detect the protein expression.Cells were treated with α-tocopheryl succinate(50 μmol/L,100 μmol/L) for 12,24 and 48 h,then the mRNA and protein expression amounts of c-IAP1 and c-IAP2 were determined by RT-PCR and western blotting.Results α-TOS significantly inhibited proliferation of MCF7 and MDA-MB-453 cells in a dose-dependent manner.The values of IC_(50) were 132 μmol/L and 74 μmol/L in MCF7 and MDA-MB-453 respectively.The expression of c-IAP1 in the levels of mRNA and proteins was inhibited by α-TOS in a dose-dependent and time-dependent manner;whereas,α-TOS has no remarkable effect on the expression of c-IAP2.We can conclude that α-TOS inhibited breast cancer cells growth by reducing the expression of c-IAP1 and is a promising anti-breast cancer drug.

Key words: a-TOS

摘要： 目的检测α-生育酚琥珀酸酯(-αTOS)对MCF7和MDA-MB-453乳腺癌细胞增殖以及细胞中凋亡抑制蛋白家族(IAPs)表达的影响。方法采用四甲基偶氮唑蓝(MTT)法测定-αTOS对人乳腺癌细胞MCF7和MDA-MB-453增殖的抑制作用,细胞分别接种于96孔板后用浓度为51、0、255、0、75、1001、25和150μmol/L的-αTOS处理48 h后检测;细胞经50μmol/L、100μmol/L-αTOS处理122、4、48 h后,用RT-PCR的方法检测细胞内c-IAP1和c-IAP2 mRNA的转录水平;用Western Blot的方法检测c-IAP1和c-IAP2的蛋白质表达水平。结果-αTOS对人乳腺癌细胞MCF7和MDA-MB-453有显著的增殖抑制作用,并表现为剂量依赖性,-αTOS对MCF7和MDA-MB-453的IC50值(细胞半数死亡的药物浓度)分别为132μmol/L和74μmol/L;RT-PCR结果显示,-αTOS使两个细胞系的c-IAP1的转录水平显著下降,且呈时间依赖性;但对c-IAP2的转录水平没有明显影响;Western Blot的结果发现-αTOS使得这两种细胞系的c-IAP1蛋白质翻译水平也呈下降趋势,该结果与mRNA转录水平下降相一致,对c-IAP2蛋白质的表达几乎无影响。因此,α-TOS可能通过抑制c-IAP1蛋白的表达而抑制MCF7和MDA-MB-453乳腺癌细胞的生长增值,a-TOS有望开发成为新的预防和治疗乳腺癌的有效药物。

关键词: -αTOS