Abstract: Objective To develop a reliable model of delayed cerebral vasospasm(DCVS) after subarachnoid hemorrhage(SAH) in rats and explore the nursing experience of the rats so as to provide experimental evidence for the study on treatment of subarachnoid hemorrhage.Methods 48 healthy adult SD rats were randomly divided into sham surgery group(Sham) and subarachnoid hemorrhage surgery(SAH) group.The surgery and two times injections of autologous intracranial blood into cisterna magna of the rats in SAH group were carried out to establish the SAH model,and the rats of sham group just had surgery without injection.At 3,5 and 7 days after the injection of the blood at the first surgery,8 rats in each group were sacrificed by the perfusion,and their basilar arteries were taken out to compare the basilar artery bore and the thickness of blood vessel wall.Results At 3,5 and 7 days after the surgery,there were significant differences in the basilar artery bore and the thickness of blood vessel wall(P<0.05) between the Sham group and the SAH group,and the cerebral vasospasm reached the peak at the seventh day.Conclusion Two times injections of autologous intracranial blood into cisterna magna is a reliable method for making DCV after SAH in rats, and the effective nursing plays an important role in successfully establishing of the model.

Key words: Subarachnoid hemorrhage, Model, Animal, Nursing, Rat

摘要： 目的制备可靠的大鼠蛛网膜下腔出血(SAH)后迟发性脑血管痉挛模型,探索模型鼠的护理经验,为蛛网膜下腔出血的治疗研究提供实验依据。方法48只健康成年Sprague-Dawley(SD)大鼠随机分为6组:对照组3组(n=8)及蛛网膜下腔出血手术3组(n=8)。采用枕大池二次注自体颅内血的方法建立SAH模型,假手术组只手术不注射,在首次手术注血后3、57、d,每组各灌注处死大鼠,取其基底动脉比较基底动脉的内口径周长和血管壁厚度。结果假手术对照组与SAH组的基底动脉的内口径周长和血管壁厚度在手术后3、57、d均有显著性差异(P<0.05),脑血管痉挛在第7 d达到高峰。术后进行了严格的护理。结论大鼠枕大池二次注自体颅内血法是可靠的SAH后迟发性脑血管痉挛模型制作方法,有效的护理对模型的成功制作极为重要。

关键词: 蛛网膜下腔出血, 模型, 动物, 护理, 大鼠