Abstract: Objective To compare the effects of the different oviduct embryo transfer methods,the periods and numbers of embryo transfer.Methods The different numbers of 1-cell embryo,2-cell embryo and,8-cell embryo were respectively transfefred into the oviduct of pseudopregnancy KM mouse,and the embryos were transfer to the pavilion of the oviduct tranfer and the oviduct ampullar transfer,respectively.Result The pregnancy rate of 2-cell embryo transfer is significant higher than 1-cell and 8-cell treatment group(66.67% vs 25%,33.33%,P<0.05);Transfer of 25 2-cell embryos via oviduct tranfer showed a higher birth rate than 15 and 35 2-cell embryos(36% vs 16.67%,10.71%,P<0.05);The pregnancy rate and birth rate of oviduct ampullar incision transfer were significant higher than pavilion of the oviduct tranfer(50% vs 75%,38.9% vs 49.3%,P<0.05).Conclusion Transfer of 25 2-cell embryos via oviduct ampullar incision transfer can improve the success rate of mouse embryo transfer.

Key words: KM mouse, Oviducts embryo transfer, 2-cell embryo, Oviduct ampullar incision transfer

摘要： 目的通过研究不同输卵管胚胎移植方法、不同细胞类型及适宜胚胎的数量对移植后妊娠率和产仔率的影响,旨在提高小鼠胚胎移植效率。方法分别将1-细胞、2-细胞和8-细胞胚胎、移入发情的假孕KM雌鼠的输卵管,选择最适宜的胚胎类型用以研究最合理的胚胎移植数量,以及比较输卵管伞移植和膨大部移植两种方法的优劣。结果2-细胞胚胎移植后的妊娠率显著高于1-细胞和8-细胞处理组(66.67%vs25%,33.33%,P<0.05);每只小鼠输卵管移植25个2-细胞胚胎后的产仔率显著高于15个和35个两处理组(36%vs16.67%,10.71%,P<0.05);采用输卵管壁切口移植的妊娠率和产仔率显著高于输卵管伞移植(50%vs75%,38.9%vs49.3%,P<0.05)。结论采用25枚左右的2-细胞期胚胎通过输卵管壁切口移植的方法可以取得较高的输卵管移植成功率。

关键词: 昆明小鼠, 输卵管移植, 2-细胞胚胎, 输卵管壁切口移植