Abstract: Objective To describe a novel method for quantifying the volume of mouse atherosclerotic lesion using the ApoE-knockout mice.Methods Frozen sections were cut from the aortic roots of the 16-year old female ApoE-knockout mice using the standardized cryosection protocol.Seven serial sections were collected at 100 μm intervals and stained with oil red-O.Lesion area and volume were analyzed and calculated using Leica Q500MC image analysis software and Cavalieri formula respectively.Results The average atherosclerotic area and lesion volume were(520 632) μm~2 and(364 442) μm~3 respectively.Conclusion The introduced method is easy to be operated and standardized.

Key words: Atherosclerosis, Mouse, Lesion area quantification, Aortic root

摘要： 目的如何对小鼠动脉粥样硬化斑块体积进行定量分析国内尚未见报到。本文将以载脂蛋白E基因敲除小鼠为例介绍一种确定小鼠动脉粥样硬化斑块体积的新方法。方法将16周龄雌性ApoE基因敲除小鼠的主动脉根部进行连续冰冻切片700μm,每间隔100μm收集组织切片进行油红O染色,利用Leica Q500MC图像分析软件测量斑块面积,利用Cavalieri公式计算动脉粥样硬化斑块体积。结果本例中小鼠主动脉根部粥样斑块面积为520 632μm2,体积为364 442μm3。结论本文介绍的确定小鼠动脉粥样硬化斑块体积的方法操作简单、易于进行标准化。

关键词: 动脉粥样硬化, 小鼠, 斑块定量, 主动脉根部