Abstract: Objective To develop a murine subcutaneous transplantation model of hepatoma with cell line Hepa1-6.Methods Hepa1-6 cell was cultured in vitro and its growth was monitored;Hepa1-6 cells were injected intradermally into C57BL/6J mice at concentrations of 2×10~5/mice(A group),2×10~6/mice(B group)and 4×10~6/mice(C group).Results The cells grew very slowly,and they went into exponential growth three days after assimilation;the rate of tumorigenesis in group A,B and C was 30%,80% and 100% respectively;Tumorigenesis was observed after 12 and 10 days in group A and B respectively,but after 7 days in group C;From the third to the forth week,the tumor bulks in group C and B grew significantly bigger than those in group A(P<0.01). There was no obvious difference in tumor bulks between Group C and B.Conclusion The murine hepatoma model can be established by subcutaneous transplantation of Hepa1-6 cells at the concentration of 4×10~6/mice.

Key words: Hepa1-6, Subcutaneous transplantation model of hepatoma

摘要： 目的培养小鼠Hepa1-6细胞,建立小鼠皮下肝癌模型,为后续实验奠定基础。方法Hepa1-6体外培养,观察细胞生长情况,按2×105/鼠(A组)、2×106/鼠(B组)和4×106/鼠(C组)的细胞量,接种于C57BL/6j小鼠,观察肿瘤生长情况。结果细胞生长速度较慢,传代第3 d为指数生长期;成瘤率分别为A组30%,B组为80%,C组为100%;A、B组分别在接种肿瘤细胞后12 d和10 d成瘤,而C组在接种肿瘤细胞后7 d成瘤,成瘤速度明显高于前两组;第3周和第4周C组和B组肿瘤生长体积显著高于A组(P<0.01),而C组和B组之间无明显差异。结论使用Hepa1-6细胞以4×106/鼠的细胞用量在SPF级雌性C57BL/6j小鼠前肢部皮下成功建立了肝癌移植瘤,完成了小鼠肝癌动物模型的建立。

关键词: Hepa1-6