Abstract: Objective To study the effect of Bushen Zhuanggu granules on bone histomorphometry in osteoporosis rats induced by retinoic acid,and discuss the mechanism of Bushen Zhuanggu granules in preventing and treating osteoporosis.Method Three-month-old SD rats were randomly divided into 5 groups according to their weight: normal control group,osteoporosis model group,low and high dose Bushen Zhuanggu granulesgroups,and Xianling Gubao-positive control group.All the rats received retinoic acid intragastrically every morning for 2 weeks,and were administered intragastrically with subject drugs for 3 weeks.Rats were anesthetized with intraperitoneal injection of 10% chloral hydrate,and sacrificed by abdominal aortic bleeding.Both tibia were taken,and treated according to the following procedure after removing the soft tissues: fixed in 10% formalin,decalcified in 10% EDTA solution,dehydrated in alcohol,embedded in paraffin,sliced,and HE stained.Percentage of trabecular bone area,trabecular thickness,trabecular bone volume and trabecular bone separation were measured and calculated at 10xl0 magnification using a high-resolution color graphic pathological analysis system,and the pathomorphological changes of upper tibia were observed.Reasult Compared with the normal control group,the model control group displayed a reduction in the percentage of bone area and thickness,and an increase in bone volume and trabecular separation in the proximal tibia cancellous bone trabecular.The highly significant difference(P<0.01) between the model control and normal control group indicated that the model was established successfully.Compared with the model control group,the Bushen Zhuanggu granules group displayed a significant increase in the percentage of bone area and thickness and a significant reduction in bone volume and trabecular separation in the proximal tibia cancellous bone trabecular(P<0.01).Conclusion Bushen Zhuanggu granules can decrease bone loss induced by retinoic acid,and improve the morphology of trabeculae,so osteoporosis can be prevented and treated.

Key words: Bushen Zhuanggu granules, Retinoic acid, Osteoporosis, Rat

摘要： 目的研究补肾壮骨颗粒对维甲酸致骨质疏松大鼠骨组织计量学的影响,探讨补肾壮骨颗粒防治骨质疏松症的机制。方法实验选用3月龄SD大鼠,按体重随机分为5组:正常对照组、骨质疏松模型组、补肾壮骨颗粒高低剂量组、仙灵骨葆阳性对照组。各组大鼠上午均灌胃给予维甲酸2周,造模同时下午按上述剂量灌胃给予受试药3周,末次给药后晚上禁食,次日以10%水合氯醛腹腔注射麻醉动物,腹主动脉放血处死动物后取左右胫骨,并去掉附着的软组织,放入10%福尔马林中固定,移入10%EDTA溶液中脱钙,再转入酒精内脱水,石蜡包埋,切片并行H-E染色。在光镜10×10倍视野下应用高清晰度彩色病理图文分析系统对胫骨上段松质骨的骨组织作静态测量并计算骨小梁面积百分数、骨小梁厚度、骨小梁数量和骨小梁分离度(取切片中心部的五个视野测量并取其平均数),观察胫骨上段病理形态学变化。结果与正常对照组相比,模型对照组大鼠的胫骨近端骨松质骨小梁面积百分数及厚度减少,骨小梁数量及分离度增加,有统计学意义(P<0.01),说明造模成功;与模型对照组相比,补肾壮骨颗粒组胫骨近端骨松质的骨小梁面积百分数及厚度增加,骨小梁数量及分离度减少,有统计学意义(P<0.01)。结论补肾壮骨颗粒能减少维甲酸通过激活破骨细胞所引起的骨丢失,改善骨小梁的形态,起到防治骨质疏松症的作用。

关键词: 补肾壮骨颗粒, 维甲酸, 骨质疏松, 大鼠