Abstract: Objective To study the effect of chronic psychological stress on ovarian function.Methods Female BALB/c mice were burdened with psychological stress by constraint.Levels of serum estradiol(E2), progesterone(P) and corticosterone(CORT) were measured after stress,and ovary-morph,the amount of primordial follicle,primary follicle,secondary follicle,etc.,pregnancy rate and subalgebra were observed.Results In the stressed mice,a significant increase of serum CORT and decrease of E2、P were found compared to those of the control group;A significant change of ovary-morph was observed;The number of vesicular graafianae grew downwards in the stressed mice(P<0.05);The number of subalgebra decreased significantly,but the pregnancy rate was not changed significantly in the stressed mice.Conclusion Chronic psychological stress can cause the disruption or degeneration of ovarian function.

Key words: Psychological stress, BALB/c mouse, Ovarian function

摘要： 目的研究长期心理应激对卵巢功能的影响。方法应用束缚的方法产生心理应激,用酶联免疫法测定心理应激BALB/C雌小鼠CORT(皮质酮),用放射免疫法测E2(雌二醇)、P(孕酮);同时观察卵巢形态、各级卵泡数及妊娠率和产子数。结果长期心理应激能使雌性小鼠CORT含量升高(P<0.05);血清E2、P的含量降低(P<0.01);卵巢形态学发生明显变化,窦前卵泡及窦卵泡数量减少(P<0.05);对小鼠妊娠率无明显影响(P>0.05),但产仔数减少,有显著差异(P<0.05)。结论长期心理应激可影响卵巢功能。

关键词: 心理应激, BALB/C小鼠, 卵巢功能