Abstract: Objective To investigate the influence of hemolysis on 14 biochemical items in SD rat blood.Methods Blood samples were collected from 20 SD rats,and using the TMS-1024 auto-biochemical analyzer,fourteen serum biochemical items were detected from each sample based on the different Hb contents,0,1,2,3,5,and 10 g/L.Results Hemolysis resulted in increasing of ALT,AST,TP,LDH,CK,TBi,and UREA levels(P<0.05/0.01) and decreasing of CR,TG,and A/G levels(P<0.05/0.01) in sera.But the levels of GLU,CHO,ALP,and ALB were not significantly affected by hemolysis(P>0.05).Conclusion A lot of biochemical items were significantly affected by hemolysis,and the levels of LDH,TG and AST are the more severely affected than others.

Key words: SD rats, Serum, Hemolysis, Biochemical test

摘要： 目的探讨标本溶血对SD大鼠14项生化检测结果的影响。方法采用TMS-1024全自动生化分析仪对20只大鼠不同溶血程度即Hb含量为0、12、、35、、10g/L的血清样本进行14项生化指标检测,并对结果进行统计学分析。结果溶血引起ALT、AST、TP、LDH、CK、TBi、UREA检测结果升高(P<0.05/0.01);引起CR、TG、A/G检测结果的降低(P<0.05/0.01);对GLU、CHO、ALP、ALB检测结果的影响不明显(P>0.05)。结论样本溶血可对较多的生化指标产生明显的影响,并且随溶血程度的增加,产生的影响越明显,其中以LDH、TG和AST最为敏感。

关键词: SD大鼠, 血清, 溶血, 生化检测