Abstract: Objective To observe the effective of Tiger skin cat on examination the depressor substance in MZR. Methods The Tiger skin cat were anesthetized and given histamine phosphate at the dose of 0.05 μg·kg~ -1 、 0.10 μg·kg~ -1 and 0.15 μg·kg~ -1 respectively by intravenous injection. Then the response value of cats' blood pressure at each dose was determined and sensitivity of cats to histamine phosphate was assessed. Histamine phosphate and MZR were injected acocording to pharmacopeia and the effects of different doses of MZR (0.05 μg·kg~ -1 、0.10 μg·kg~ -1 、0.15 μg·kg~ -1 ) on cats' blood pressure were observed. Results The value of cats' blood pressure exceeded 2.67 kPa after different doses of histamine phosphate injection and the sensitivity of cats to histamine phosphate was qualification. The changes of cats' blood pressure in different MZR were not greater then a half of that induced by histamine phosphate. Conclusions The MZR depressor substance was consistent with pharmacopeia and the Tiger skin cats could be used for the examination of the depressor substance in MZR.

Key words: Tiger skin cat, Mizoribine, Depressor substance

摘要： 目的考查虎皮猫用于测定咪唑立宾(MZR)降压物质的效果。方法虎皮猫麻醉后,依次按0.05μg·kg-1、0.10μg·kg-1、0.15μg·kg-1静脉注射磷酸组织胺对照品,测定各剂量磷酸组织胺对猫血压所致的反应值(注射各剂量前后血压的改变幅度),判定猫对磷酸组织胺降压反应的灵敏度。按药典规定方法静脉注射磷酸组织胺和咪唑立宾,观察咪唑立宾在15mg·kg-1、30mg·kg-1、40mg·kg-1剂量下,对猫血压的影响。结果虎皮猫注射不同剂量磷酸组织胺后,血压反应均超过2.67kPa,灵敏度测定合格。每批MZR各剂量dT所致的反应值均不大于ds所致的反应值的一半。结论咪唑立宾降压物质检查符合药典要求,虎皮猫可用于MZR降压物质的检查。

关键词: 虎皮猫, 咪唑立宾, 降压物质