Abstract: Objective To establish embryo bank for Smad2 gene knock-out mice. Methods The OPS vitrification was used to cryopreservation of morula and blastocyst embryos of the Smad2 gene knock-out mice.We compare the results of superovulatory treatment,recovery rate and survival rate in different groups.Results The average numbers of embryo production of Smad2~(+/-) and Smad2~(+/+) were 14.13 and 24.60 respectively after superovulatory treatment.The recovery rates of the two group embryos were 90.16% and 91.67% respectively.The rates of development were 73.08% and 77.05% in vitro respectively after thawing.The results indicated that the numbers of superovulatory of Smad2~(+/-) were lower than the ones of Smad2~(+/+),the rates of recovery and the rate of survival were no different between the two group mouse after thawing.We have cryopreserved 1256 embryos of the Smad2 gene knock-out mice by OPS vitrification.Conclusion We establish the embryo bank for the Smad2 gene knock-out mice.

Key words: Smad2, Knockout mice, Embryo, Vitrification

摘要： 目的建立Smad2基因敲除小鼠胚胎库。方法利用OPS法对Smad2基因敲除小鼠胚胎进行玻璃化冷冻保存,并比较不同杂交组合小鼠的超数排卵数、解冻胚胎的复苏率及发育率。结果两组不同杂交组合(Smad2+/-♂×Smad2+/-♀和Smad2+/-♂×Smad2+/+♀)小鼠平均超排卵数分别为14.13枚和24.60枚;复苏率分别为90.16%和91.67%;囊胚发育率分别为73.08%和77.05%。这些结果表明Smad2基因敲除杂合子母鼠的超排数量明显低于野生型母鼠的超排数量,而二者胚胎解冻后的复苏率和囊胚发育率没有显著差异。因此我们主要通过对野生型小鼠超数排卵,然后与Smad2基因敲除杂合子雄鼠交配的方法获取胚胎,进行玻璃化冷冻保存,现已冻存胚胎1256枚。结论成功建立了Smad2基因敲除小鼠胚胎库。

关键词: Smad2, 基因敲除小鼠, 胚胎, 玻璃化冷冻