Abstract: Objective To establish and compare the typeⅡdiabetes mellitus models in three strains of native minipigs for promoting their use on type Ⅱdiabetes mellitus research.Methods Six Bama minipigs,six Wuzhishan minipigs and six Nongda minipigs were fed with a high-sucrose high-fat diet to induce type Ⅱ diabetes mellitus.Four Nongda minipigs were assigned as controls fed with a normal diet.Body weight,fasting serum glucose and insulin concentrations were measured monthly,and serum TG,CHO,HDL-C,LDL-C and intravenous glucose tolerance test(IVGTT) were tested at 2 month intervals during the 8-month inducing period.Results Bama minipigs and Wuzhishan minipigs were susceptible to the high-sucrose high-fat diet.Obesity,hyperinsulinemia and impaired insulin tolerance were noted at month 5 and 6.One of six Bama minipigs and one of six Wuzhishan minipigs developed diabetes mellitus and three Bama minipigs and two Wuzhishan minipigs reached the pre-diabetes stage at month 8.Nongda minipigs were relatively resistant to the high-sucrose high-fat diet.Fasting serum glucose,insulin concentration,insulin sensitivity index and IVGTT remained normal during the 8 months high-sucrose high-fat feeding.Only slightly obesity and despaired insulin tolerance were observed at month 7 and 8.All the parameters related to blood lipid metabolism(TG,CHO,HDL-C,LDL-C) were increased significantly after feeding high-sucrose and high-fat in the three strains of minipigs with Bama minipigs increased more significantly.Conclusion Bama minipigs and Wuzhishan minipigs were susceptible to the high-sucrose and high-fat diet inducing typeⅡdiabetes millitus.Susceptibility differences existed among the animals in the same strains.Nongda minipigs were less susceptible to the dietary inducing.The results provided foundamental data for further research on typeⅡdiabetes mellitus susceptibility relative genes in the minipigs and for selective breeding of family stocks with high typeⅡdiabetes mellitus susceptibility.

Key words: Minipig, TypeⅡdiabetes mellitus, Animal model, Bama minipig, Wuzhishan minipig

摘要： 目的比较三个品系小型猪对高糖高脂饲料诱发Ⅱ型糖尿病模型的敏感性,为我国小型猪在Ⅱ型糖尿病研究中的应用提供基础资料。方法4~5月龄巴马小型猪、五指山小型猪和中国农大小型猪各6头,以4头中国农大小型猪为对照,同步开展高糖高脂饲料喂养试验,试验周期8个月。每月称1次体重,测定血糖、血清胰岛素含量,每2个月进行1次静脉葡萄糖耐量试验(IVGTT),测定脂质代谢相关指标。结果巴马小型猪和五指山小型猪对高糖、高脂饲料比较敏感,在饲喂5~6个月后,开始出现糖代谢异常,表现为显著肥胖、血清胰岛素升高和胰岛素敏感性下降。至实验后8个月,巴马小型猪和五指山小型猪各有1头发展为糖尿病,分别有3头和2头出现糖尿病前期症状。中国农大小型猪对高糖高脂饲料诱发糖尿病相对不敏感,实验期间空腹血糖水平、血清胰岛素含量、胰岛素敏感指数和IVGTT一直无显著变化,仅在实验后7个月开始表现肥胖,8个月出现胰岛素敏感性减低。三个品系小型猪在高糖高脂饲喂后,血脂相关指标(TG,CHO,HDL-C,LDL-C)普遍升高,以巴马小型猪升高幅度最大。结论巴马小型猪和五指山小型猪对高糖、高脂饲料诱发Ⅱ型糖尿病比较敏感,品系内个体差异较大。中国农大小型猪相对不敏感。这一结果为进一步的Ⅱ型糖尿病相关基因分析和高敏感家系培育研究提供了基础资料。

关键词: 小型猪, Ⅱ型糖尿病, 动物模型, 五指山小型猪