The Application of BLS Cell Fusion Instrument on Porcine Parthenogenetic Activation

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The Application of BLS Cell Fusion Instrument on Porcine Parthenogenetic Activation

LIU Xiao~

(1,2),ZHANG Qing-qiao~3,PAN Deng-ke~(2*),CHEN Kou-kou~(2,4),ZHANG Wei-hong~(2,4),FENG Chong~(2,4),FENG Shu-tang~2(1.Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences(CAAS),Lanzhou 730050)(2.Institute of Animal Science,CAAS,Beijing 100094)(3.Hebei University of Engineering,Handan 056038,Hebei Province)(4.Gansu Agricultural University,Lanzhou 730070

Received:2007-12-28 Revised:2007-12-28 Online:2007-12-28 Published:2007-12-28

电融合仪BLS在猪卵母细胞孤雌激活中的应用

刘晓;张庆桥;潘登科;陈扣扣;张卫红;冯冲;冯书堂;

中国农业科学院兰州畜牧与兽药研究所,河北工程大学,中国农业科学院北京畜牧兽医研究所,中国农业科学院北京畜牧兽医研究所,中国农业科学院北京畜牧兽医研究所,中国农业科学院北京畜牧兽医研究所,中国农业科学院北京畜牧兽医研究所兰州730050,中国农业科学院北京畜牧兽医研究所,北京100094,河北邯郸056038,北京100094,北京100094,甘肃农业大学,兰州730070,北京100094,甘肃农业大学,兰州730070,北京100094,甘肃农业大学,兰州730070,北京100094

Abstract

Abstract: Objective To establish and optimize the electric parameters of BLS cell fusion on porcine parthenogenetic embryo,being for the cloned animals.Methods MⅡ oocytes matured in vitro were activated by electrically stimulation with different pulses and different strength and different field duration to induce parthenogenetic activation and further development.Results\ Parameter (1.2 kV/cm,100 μs,2 pulses) supported higher percentages of cleavage and blastocyst(70% and 30% or so);BLS fusion instrument was efficient and stable for production of porcine embryos in vitro.

Key words: Porcine, Parthenogenetic activation, BLS fusion instrument

摘要： 目的建立并优化BLS细胞融合仪在猪孤雌胚体外生产中的使用条件,同时为克隆胚的生产提供数据参考。方法以体外成熟的猪卵母细胞为研究对象,研究了不同的电场强度、脉冲时程和脉冲次数对猪卵母细胞电激活效果及其对孤雌胚发育率的影响。结果电激参数(1.2 kV/cm、100μs、2次脉冲)能够得到较高的卵裂率和囊胚率(70%、30%左右);BLS细胞融合仪是一种经济高效的胚胎激活仪。

关键词: 猪, 孤雌激活, BLS融合仪

LIU Xiao~. The Application of BLS Cell Fusion Instrument on Porcine Parthenogenetic Activation[J]. laboratory animal science.

刘晓;张庆桥;潘登科;陈扣扣;张卫红;冯冲;冯书堂;. 电融合仪BLS在猪卵母细胞孤雌激活中的应用[J]. 实验动物科学.

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The Application of BLS Cell Fusion Instrument on Porcine Parthenogenetic Activation

电融合仪BLS在猪卵母细胞孤雌激活中的应用

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