The Determination of Blood Physiological Indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) Transgenic Mice of Different Ages

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The Determination of Blood Physiological Indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) Transgenic Mice of Different Ages

WANG Dong-ping~1,SUI Li-hua~1,WU Na~1,SUN Yan-song~

(1**),KONG De-qiang~1,SHANG Shu-jiang~1,LI Wen-long~1,SHANG Shi-chen~1,LI Mu~1,LI Gui-jun~1,FAN Xiu~1,ZHANG Ai-lan~1,YANG Xiao~2(1.Laboratory Animal Center,Chinese Academy of Military Medical Sciences,Beijing 100072)(2.Development and Genetic Diseases Department of Bioengineering Institute,Chinese Academy of Military Medical Sciences,Beijing 100071

Received:2007-10-28 Revised:2007-10-28 Online:2007-10-28 Published:2007-10-28

p21~(HBsAg/HBsAg)和p21~(HBX/HBX)转基因小鼠不同月龄血液生理指标的测定

王冬平;隋丽华;吴娜;孙岩松;孔德强;尚书江;李文龙;尚世臣;李慕;李桂军;凡秀;张爱兰;杨晓;

军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院生物工程研究所发育和疾病遗传学研究室北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100072,北京100071

Abstract

Abstract: Objective To determine blood physiological indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) transgenic mice of different ages so as to delve further into the effect of HBsAg/HBsAg and HBX/HBX transferred into p21 locus of mice on their blood physiological indexes. Methods The blood physiological indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) transgenic mice of different ages were determined and the results were compared with those of wild mice.Results The differences in many blood physiological indexes between male and female mice were very significant,and the differences in blood physiological indexes between p21~(HBsAg/HBsAg),p21~(HBX/HBX) transgenic mice and wild mice were very significant.Conclusion The transferring of HBsAg/HBsAg and HBX/HBX gene into p21 locus of mice could exert the effect on their blood physiological indexes.

Key words: Transgenic mice, Blood physiological indexes

摘要： 目的检测p21HBsAg/HBsAg和p21HBX/HBX转基因小鼠的血液生理指标,分析p21HBsAg/HBsAg和p21HBX/HBX基因引入小鼠基因组p21位点中对其血液指标的影响。方法用日本光电MEK-5126K血球计数仪(动物芯片)及配套试剂盒对四个月龄不同基因型小鼠的血液生理指标进行测定,并且与野生型对照组进行比较。结果多数指标雌雄之间差异显著,并且两种基因型小鼠及野生型在不同年龄、不同指标的动态变化不同。结论p21HBsAg/HBsAg和p21HBX/HBX基因转入小鼠基因组p21位点对其血液生理指标具有一定的影响。

关键词: 转基因小鼠, 血液生理指标

WANG Dong-ping~1,SUI Li-hua~1,WU Na~1,SUN Yan-song~. The Determination of Blood Physiological Indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) Transgenic Mice of Different Ages[J]. laboratory animal science.

王冬平;隋丽华;吴娜;孙岩松;孔德强;尚书江;李文龙;尚世臣;李慕;李桂军;凡秀;张爱兰;杨晓;. p21~(HBsAg/HBsAg)和p21~(HBX/HBX)转基因小鼠不同月龄血液生理指标的测定[J]. 实验动物科学.

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The Determination of Blood Physiological Indexes of p21~(HBsAg/HBsAg) and p21~(HBX/HBX) Transgenic Mice of Different Ages

p21~(HBsAg/HBsAg)和p21~(HBX/HBX)转基因小鼠不同月龄血液生理指标的测定

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