Abstract: Objective To develop the model of drug-dependent constipation in rats.Methods The constipation was induced in the rats by ad lib food and limited water-intake.Then the rats with constipation were treated by oral administration of phenothalin solution and rhubarb soak at the initial dose of 20 mg/100 g/d.The dose was increased daily by 20 mg/100 g,with the final dose of 420 mg/100 g/d and 320 mg/100 g/d,respectively.The drugs were continuously given at the final dose for three months to induce drug-dependent constipation.The intestinal transit functions of the rats,such as small intestine advance rate,stools quantity and dry weight,time of first having stools,phenol red content in stools,and number of red small plastic ball in stools,were observed in both normal and model groups.Results As compared with the rats of the normal group,the small intestine advance rate was slow,the time of first having stools was laggard,and stool quantity and phenol red content in stools were decreased in the rats of the model group.Conclusion The model of drug-dependent constipation was successfully developed in the rat, which was simple,economical,and reproducible.

Key words: Rat, Drug-dependent constipation, Animal model

摘要： 目的建立一种大鼠药物依赖性便秘的动物模型。方法不禁食,限水,致大鼠形成便秘;然后用大黄,酚酞灌胃给予治疗,起始剂量每天20 mg/100 g/d,按每天20 mg/100 g/d递增;大鼠对药物产生依赖后,再逐渐停止限水,大黄最终用量320 mg/100 g/d,酚酞最终用量420 mg/100 g/d,再维持此剂量不变饲养三个月。观察实验动物大鼠肠道的传输功能:粪便排出量,粪便的干重,首粒大便排出时间,大便内酚红含量,大便中红色塑料小球排出情况,小肠推动率。结果与正常对照组比较,各模型组小肠推动率减慢,首粒大便排出时间长,排便量少,大便内酚红含量减少。结论成功地建立了一种大鼠药物依赖性便秘的动物模型,该模型简单经济,可重复性强。

关键词: 大鼠, 药物依赖性便秘, 动物模型