Abstract: Objective To observe the changes of splenic total T lymphocytes and T lymphocyte subgroups during immunologic rejection after allogeneic and heterogenic peripheral nerve grafting so as to delve further into the possibility of the repair of nerve defection.Methods BALB/c mice were divided into sham-operation group,fresh autograft group,fresh allogeneic nerve graft group,fresh heterogenic nerve graft group,chemical de-cell allogeneic nerve graft group and chemical de-cell heterogenic nerve graft group.The different treated nerve grafts were put into the thigh muscle pocket of the mice.The mice were sacrificed respectively on the third,seventh,fourteenth,twenty-eighth postoperative day,and the spleens were sampled.The T lymphocyte counting was carried out and CD3+,CD4+,CD8+,and CD25+cells were measured with the flow cytometer.Results The differences in percentages of CD3+,CD4+,CD8+,and CD25+cells between chemical de-cell allogeneic nerve graft group,chemical de-cell heterogenic nerve graft group and sham-operation group,fresh autograft group on the seventh,fourteenth,twenty-eighth postoperative day were not significant statistically(P>0.05).The percentages of CD3+,CD4+,CD8+,and CD25+cells in fresh allogeneic nerve graft group and fresh heterogenic nerve graft group were much higher than in sham-operation group and fresh autograft group,and the differences were significant statistically(P<0.05).Conclusion The immunogenicities of chemical de-cell allogeneic peripheral nerve and chemical de-cell heterogenic peripheral nerve are very similar to that of self-peripheral nerve,and are much lower than those of fresh allogeneic peripheral nerve and fresh heterogenic peripheral nerve.

Key words: Peripheral nerve graft, T Lymphocyte subgroups, Immunogenicity

摘要： 目的通过化学法萃取的去细胞异体及异种神经移植后,观察移植免疫排斥过程中小鼠脾脏T细胞总数与亚群变化,探讨周围神经缺损修复的可行性。方法BALB/c小鼠随机分成假手术组、新鲜自体神经移植组、新鲜同种异体神经移植组、新鲜异种神经移植组、化学去细胞同种异体神经移植组和化学去细胞异种神经移植组,每组32只。分别在神经移植术后3、71、4、28 d,每组各取8只小鼠脾脏,淋巴细胞分离液获取淋巴细胞,计数。用荧光标记的CD3+、CD4+、CD8+、CD25+单抗作用后,流式细胞仪检测T淋巴细胞及其亚群百分数,Stata7.0统计软件进行统计处理。结果在7 d1、4 d、28 d各时间点上,化学去细胞神经同种异体和异种神经移植组CD3+、CD4+、CD8+、CD25+阳性T细胞百分率与假手术对照组、自体神经移植组相比无显著差异(P>0.05),而新鲜同种异体及异种神经移植组在相同时间点上的CD3+、CD4+、CD8+、CD25+阳性T细胞百分率与假手术对照组、自体神经移植组相比则有明显增高,差异显著(P<0.05)。结论新鲜同种异体周围神经移植后的排斥属于急性排斥反应。化学去细胞处理的同种异体神经的免疫原性等于或接近于自体神经,要明显低于新鲜同种异体神经移植。

关键词: 周围神经神经移植, T细胞亚群, 免疫原性, 流式细胞术