Abstract: Objective By comparing the pessary and the microscopic smear for predicting the accuracy of pregnancy to improve rat model manufacture method.Method 300 Wistar rats were put together in cages for 12 h in which the femela/male ratios were 2∶1.At the same time two methods were used to examine the pregnant situation of rats.Results The masculine gender rate of the pessary method was 72.5%,the false masculine gender rate was 2.06% and the false negative rate was 5.23%.The masculine gender rate of the microscope smear method was 43.5%,the false masculine gender rate was 0,the false negative rate was 43.1%.Conclusion The accuracy rate of the pessary is higher than the microscope smear.

Key words: Animal model, Pregnancy, Rat

摘要： 目的比较观察阴栓和显微镜涂片检测大鼠受孕的准确性,改进大鼠模型制作方法。方法Wistar大鼠300只,以雌雄比例2∶1合笼12 h,两种方法同时检测大鼠受孕情况,至确认大鼠受孕,计算两种方法的阳性率并加以比较。结果阴栓法阳性率为72.5%,假阳性率为2.06%,假阴性率为5.23%;显微镜涂片法阳性率为43.5%,假阳性率为0,假阴性率为43.1%。结论检测阴栓的准确率较显微镜涂片高。

关键词: 动物模型, 妊娠, 大鼠