Abstract: Objective To study the effect on peripheral blood parameters of different lightingregimens in BALA/c nude mice.Method The BALB/c nude mice were divided into three groups:(16 h bright 8 h dark),normal illumination group(12 h bright 12 h dark),and short illumination group(8 h bright 16 h dark).The animals were maintained in three different lightingregiments,to produce second generation to 70 days old,then the peripheral blood parameters were measured,including 15 indexes altogether.Result In the long illumination group and the short illumination group,compared with the normal illumination time group,the hemoglobin determination(HGB) and the red blood cell pressure accumulates(HCT) were both lower.In the long illumination group,compared with the normal illumination group,MTD and the GRA quantity were higher.The short illumination group compared with the normal illumination group,GRA% were lower and LYM% were higher,and PLT were higher.Conclusion The different lightingregiment can create the certain disturbance to the mouse blood corpuscle,normal illumination creates the disturbance is smaller,and suits mouse maintenance.

Key words: Lightingregimen, BALB/c nude mice

摘要： 目的考察不同光照制对BALB/c裸小鼠血细胞的影响。方法将BALB/c裸小鼠分成三组:长光照组(16 h光照8、h黑暗)、正常光照组(12 h光照1、2 h黑暗)、短光照组(8 h光照1、6 h黑暗),置于不同光照环境中饲养繁殖,产生下一代,饲养至70日龄测定血细胞常规,共15项指标。结果长光照组、短光照组与正常光照时间组比较,血红蛋白(HGB)及红细胞压积(HCT)两项都明显偏低;长光照组与正常光照组比较,中间细胞总数(MTD)、中性粒细胞(GRA)数量偏高;短光照组与正常组比较,GRA%偏低,淋巴细胞百分比(LYM%)偏高,PLT偏高。讨论不同光照制对BALB/c裸小鼠血细胞会造成一定的干扰,正常光照造成的干扰较小,适合BALB/c裸小鼠饲养。

关键词: 光照制, BALB/c裸小鼠, 血细胞