Abstract: Objective To probe into the outlines of application and reproduction of animal model of Alzheimer's disease caused by lesion of the basal forebrain cholinergic neuron and to introduce the development of comparative biology of the model.Methods The animal model was developed by injection of Ibotenic acid in nucleus basalis of Meynert,and the abilities of learning and memory were evaluated by water maze.Results This treatment could induce the decline in abilities of learning and memory in the operated rats,however,there was marked different between individuals with variation coefficient from 27.1% to 46.2%.Thus 95% confidence interval of the score in water maze was selected as a standard to filtrate the animal model.The eligibility rate was 50% to 70%,which indicated that it was important filtrate the animal model after the reproduction.Conclusion the developed animal model by lesion of basal forebrain cholinergic neuron could play an important role in studying Alzheimer's disease,but the attention should be paid to the methodological aspect in the practice.

Key words: Alzheimer's disease, Animal model, Lesions of the basal forebrain cholinergic neuron

摘要： 目的介绍一种老年性痴呆的方法学动物模型-基底前脑胆碱能神经元损毁模型的复制及应用要点,以及该模型比较生物学研究的有关进展。方法采用鹅膏覃氨酸基底前脑区Maynert基底核定位注射造模,通过水迷宫实验测试动物的学习记忆能力。结果该手术方法能造成动物学习记忆能力下降,但各组内动物个体差异极大,变异系数为27.1%~46.2%。以正常动物平均成绩的95%置信区间为标准,对造模的成功率进行筛选,表明该模型的成功率为50%~75%,说明了进行模型筛选的重要意义。结论基底前脑胆碱能神经元损毁模型对老年性痴呆研究具有重要价值,但在应用中需要注意有关方法学问题。

关键词: 老年性痴呆, 动物模型, 胆碱能神经元损毁