Abstract: Objective\ To establish PCR test method that monitor Lymphocytic Choriomeningitis Virus in Laboratory.Methods Design two series primers of Nested PCR according to S segment(GPC and NP genes) of LCMV cDNA.To clone and measure sequence for our LCMV strain though PCR.Use Nested PCR to test 15 mice brains.Results That is 99% homology between the target segment and LCMV cDNA.The test result of mice brains PCR is 10 positive and 5 negative.Conclusion The test result is correct on LCMV infected mice brains,therefore,it indicates the Nested_PCR method can be used for testing LCMV in Laboratory.

Key words: LCMV, Nested-PCR, Mouse brain

摘要： 目的建立检测淋巴细胞脉络丛脑膜炎病毒(LCMV)的PCR方法。方法根据LCMV CDNA S片段的GPC和NP基因设计合成4对引物,对标准毒株进行套式PCR扩增并克隆测序,并对15份已知阳性或阴性小鼠鼠脑进行检测。结果目的片断与LCMV cDNA序列99%同源,对鼠脑的检测结果为10只阳性,5只阴性。结论检测小鼠鼠脑LCMV的结果准确,证明套式PCR能够应用于LCMV的实验室检测。

关键词: 淋巴细胞脉络丛脑膜炎病毒, 套式PCR